树突状细胞特异性细胞间黏附分子3结合非整合素在人外周血单核细胞来源的树突状细胞上的表达
Expression of dendritic cell-specific intercellular adhesion molecule 3 grabbing nonintegrin on dendritic cells generated from human peripheral blood monocytes.
作者信息
Li Jun, Feng Zhi-Hua, Li Guang-Yu, Mou Dan-Lei, Nie Qing-He
机构信息
The Center of Diagnosis and Treatment for Infectious Diseases, Tangdu Hospital, Fourth Military Medical University, Xi'an 710038, Shaanxi Province, China.
出版信息
World J Gastroenterol. 2006 Jan 21;12(3):453-6. doi: 10.3748/wjg.v12.i3.453.
AIM
To generate dendritic cells (DCs) from human peripheral blood and to detect the expression of dendritic cell-specific intercellular adhesion molecule 3 grabbing nonintegrin (DC-SIGN; CD209) for the further study of DC-SIGN in hepatitis C virus (HCV) transmission.
METHODS
Peripheral blood monocytes were isolated from blood of healthy individuals by Ficoll-Hypaque sedimentation and cultured in complete medium containing rhGM-CSF and rhIL-4. Cells were cultured for seven days, with cytokine addition every two days to obtain immature DCs. Characteristics of the cultured cells were observed under light and scanning microscope, and the expression of DC-SIGN was detected by immunofluorescence staining.
RESULTS
After seven-day culture, a large number of cells with typical characteristics of DCs appeared. Their characteristics were observed under light and scanning electron microscope. These cells had a variety of cell shapes such as those of bipolar elongate cells, elaborate stellate cells and DCs. DC-SIGN was detected by immunofluorescence staining and its expression level on cultivated dendritic cells was high.
CONCLUSION
DCs with a high expression of DC-SIGN can be generated from human peripheral blood monocytes in complete medium containing rhGM-CSF and rhIL-4.
目的
从人外周血中生成树突状细胞（DCs），并检测树突状细胞特异性细胞间黏附分子3结合非整合素（DC-SIGN；CD209）的表达，以便进一步研究DC-SIGN在丙型肝炎病毒（HCV）传播中的作用。
方法
通过Ficoll-Hypaque沉降法从健康个体的血液中分离外周血单核细胞，并在含有重组人粒细胞巨噬细胞集落刺激因子（rhGM-CSF）和重组人白细胞介素-4（rhIL-4）的完全培养基中培养。细胞培养7天，每两天添加一次细胞因子以获得未成熟的DCs。在光学显微镜和扫描显微镜下观察培养细胞的特征，并通过免疫荧光染色检测DC-SIGN的表达。
结果
经过7天培养，出现了大量具有DCs典型特征的细胞。在光学显微镜和扫描电子显微镜下观察了它们的特征。这些细胞具有多种细胞形态，如双极伸长细胞、精致的星状细胞和DCs。通过免疫荧光染色检测到DC-SIGN，并且其在培养的树突状细胞上的表达水平很高。
结论
在含有rhGM-CSF和rhIL-4的完全培养基中，人外周血单核细胞可生成高表达DC-SIGN的DCs。
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