Hultgren O H, Berglund M, Bjursten M, Hultgren Hörnquist E
Department of Clinical Immunology, Göteborg University, Guldhedsgatan 10 A, S-413 46 Göteborg, Sweden.
World J Gastroenterol. 2006 Jan 28;12(4):621-4. doi: 10.3748/wjg.v12.i4.621.
To study the serum concentration of IL-1beta, IL-1 receptor antagonist (IL-1Ra) and IL-18 in Galphai2-deficient mice at the age of 6 (healthy), 12 (pre-colitic) and 24 wk (colitic) and in healthy control mice.
At the time of killing, serum samples were collected and IL-1beta, IL-1Ra and IL-18 levels were measured using enzyme-linked immunosorbent assays.
Serum concentration of IL-1Ra was significantly increased in pre-colitic (median: 524 ng/L; P=0.02) and colitic (450 ng/L; P=0.01), but not in healthy (196 ng/L) Galphai2-deficient mice as compared with controls (217 ng/L). Serum concentrations of IL-1beta did not differ between Galphai2-deficient mice and their controls, irrespective of age, IL-18 was significantly increased in colitic, but not in pre-colitic mice compared with controls (510 ng/L vs 190 ng/L; P=0.05).
The increased serum concentrations of IL-18 and IL-1Ra in established diseases are suggested as markers of ongoing colitis. Interestingly, the significantly increased serum concentration of IL-1Ra in pre-colitic mice is found to be an early marker of disease progression.
研究Gαi2基因缺陷小鼠在6周龄（健康）、12周龄（结肠炎前期）和24周龄（结肠炎期）时以及健康对照小鼠血清中白细胞介素-1β（IL-1β）、白细胞介素-1受体拮抗剂（IL-1Ra）和白细胞介素-18（IL-18）的浓度。
处死小鼠时采集血清样本，采用酶联免疫吸附测定法检测IL-1β、IL-1Ra和IL-18水平。
与对照小鼠（217 ng/L）相比，结肠炎前期（中位数：524 ng/L；P = 0.02）和结肠炎期（450 ng/L；P = 0.01）的Gαi2基因缺陷小鼠血清中IL-1Ra浓度显著升高，但健康（196 ng/L）的Gαi2基因缺陷小鼠血清中IL-1Ra浓度与对照小鼠无差异。无论年龄如何，Gαi2基因缺陷小鼠与其对照小鼠血清中IL-1β浓度均无差异；与对照小鼠相比，结肠炎期小鼠血清中IL-18显著升高，但结肠炎前期小鼠血清中IL-18无显著升高（510 ng/L对190 ng/L；P = 0.05）。
在已确诊疾病中血清IL-18和IL-1Ra浓度升高被认为是结肠炎持续存在的标志物。有趣的是，发现结肠炎前期小鼠血清中IL-1Ra浓度显著升高是疾病进展的早期标志物。
