肠炎沙门氏菌兽医分离株中marA、soxS和acrB的过表达很少与环己烷耐受性相关。
Overexpression of marA, soxS and acrB in veterinary isolates of Salmonella enterica rarely correlates with cyclohexane tolerance.
作者信息
Webber Mark, Buckley Anthony M, Randall Luke P, Woodward Martin J, Piddock Laura J V
机构信息
Antimicrobial Agents Research Group, Division of Immunity and Infection, University of Birmingham, UK.
出版信息
J Antimicrob Chemother. 2006 Apr;57(4):673-9. doi: 10.1093/jac/dkl025. Epub 2006 Feb 21.
OBJECTIVES
To determine the contribution of the AcrAB efflux system to cyclohexane tolerance in Salmonella enterica.
METHODS
The expression of the efflux pump gene, acrB, and regulators marA and soxS from 46 isolates of S. enterica of 14 different serovars was determined by comparative RT-PCR and denaturing HPLC analysis.
RESULTS
Twenty-one of the 46 isolates were cyclohexane tolerant, a phenotype associated with multiple antibiotic resistance (MAR) and overexpression of efflux pumps. Of the cyclohexane-tolerant isolates 81% were MAR, whereas only 44% of the cyclohexane-susceptible isolates were MAR, confirming the association between cyclohexane tolerance and MAR. However, there was no correlation between cyclohexane tolerance or MAR and overexpression of acrB, soxS or marA.
CONCLUSIONS
These data suggest that cyclohexane tolerance in S. enterica can be mediated by an acrB-independent mechanism.
目的
确定AcrAB外排系统对肠炎沙门氏菌环己烷耐受性的作用。
方法
通过比较逆转录聚合酶链反应（RT-PCR）和变性高效液相色谱分析，测定了来自14种不同血清型的46株肠炎沙门氏菌外排泵基因acrB以及调节基因marA和soxS的表达。
结果
46株分离株中有21株对环己烷耐受，该表型与多重抗生素耐药性（MAR）以及外排泵的过表达相关。在对环己烷耐受的分离株中，81%具有多重抗生素耐药性，而在对环己烷敏感的分离株中，只有44%具有多重抗生素耐药性，这证实了环己烷耐受性与多重抗生素耐药性之间的关联。然而，环己烷耐受性或多重抗生素耐药性与acrB、soxS或marA的过表达之间没有相关性。
结论
这些数据表明，肠炎沙门氏菌对环己烷的耐受性可能由一种不依赖acrB的机制介导。
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