National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, South China Agricultural University, Guangzhou, China.
Laboratory of Veterinary Pharmacology, College of Veterinary Medicine, South China Agricultural University, Guangzhou, China.
Front Cell Infect Microbiol. 2019 Jul 3;9:242. doi: 10.3389/fcimb.2019.00242. eCollection 2019.
serotype Typhimurium is a major global food-borne pathogen and causes life-threatening infections. Although the resistance mechanisms to fluoroquinolones in . Typhimurium had been well-defined, tolerance to fluoroquinolones and the associated mechanism for this are obscure. In the current work, we investigated an -positive plasmid pHXY0908 and analyzed its role in . Typhimurium tolerance to ciprofloxacin using time-kill, transcriptome sequencing and real-time PCR. . Typhimurium ATCC14028 could survive under lethal concentrations of ciprofloxacin after acquiring plasmid pHXY0908. Transcriptome sequence analysis showed the chromosomal genes were systematically regulated after acquiring this plasmid suggesting an interaction between chromosome and plasmid. Additionally, the chromosomal efflux pump genes , and were up-regulated after acquiring plasmid pHXY0908 suggesting that these efflux pumps may contribute to the survival of ATCC14028 exposed to the lethal concentrations of ciprofloxacin. In conclusion, this is the first known report demonstrating that an IncHI2 type plasmid harboring could assist . Typhimurium survival under lethal concentrations of ciprofloxacin.
血清型鼠伤寒沙门氏菌是一种主要的全球食源性病原体，可引起危及生命的感染。尽管鼠伤寒沙门氏菌对氟喹诺酮类药物的耐药机制已得到很好的定义，但对氟喹诺酮类药物的耐受性及其相关机制尚不清楚。在本研究中，我们研究了一个阳性质粒 pHXY0908，并通过时间杀伤、转录组测序和实时 PCR 分析了其在鼠伤寒沙门氏菌对环丙沙星耐受性中的作用。鼠伤寒沙门氏菌 ATCC14028 在获得质粒 pHXY0908 后能够在环丙沙星的致死浓度下存活。转录组序列分析表明，获得该质粒后，染色体基因被系统调节，提示染色体和质粒之间存在相互作用。此外，获得质粒 pHXY0908 后，染色体上的外排泵基因 和 上调，表明这些外排泵可能有助于 ATCC14028 在暴露于环丙沙星的致死浓度下存活。总之，这是第一个已知的报告，证明携带 的 IncHI2 型质粒可以帮助鼠伤寒沙门氏菌在环丙沙星的致死浓度下存活。
