Chattopadhyay S K, Sengupta D N, Fredrickson T N, Morse H C, Hartley J W
Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
J Virol. 1991 Aug;65(8):4232-41. doi: 10.1128/JVI.65.8.4232-4241.1991.
LP-BM5 murine leukemia virus, a derivative of Duplan-Laterjet virus, contains a mixture of replication-competent B-tropic ecotropic and mink cell focus-inducing (MCF) viruses and a defective genome that is the proximal cause of a syndrome, murine AIDS (MAIDS), characterized by lymphoproliferation and immunodeficiency. The defective (BM5d) and ecotropic components of this mixture were molecularly cloned, and complete (BM5d) or partial (ecotropic) nucleotide sequences were determined. BM5d closely resembled the Du5H genome cloned from the Duplan virus, featuring a highly divergent p12 sequence in the gag open reading frame. In MAIDS-sensitive C57BL/6 mice, BM5d was detected in tissues within 2 weeks of infection but was absent from tissues of the MAIDS-resistant strain, A/J, 12 weeks after infection. B-cell-lineage tumors from mice with MAIDS contained and expressed BM5d, and clonal integrations of this genome were variably associated with clonal expansions of B cells in infected mice. Finally, mRNA crosshybridizing with a probe for BM5d was present in spleen but not kidney cells of uninfected B6 mice.
LP - BM5鼠白血病病毒是Duplan - Laterjet病毒的衍生物，包含具有复制能力的B嗜性亲嗜性和貂细胞集落诱导（MCF）病毒的混合物以及一种缺陷基因组，该缺陷基因组是一种以淋巴细胞增殖和免疫缺陷为特征的综合征——鼠艾滋病（MAIDS）的近端病因。对该混合物中的缺陷（BM5d）和亲嗜性成分进行了分子克隆，并测定了完整（BM5d）或部分（亲嗜性）核苷酸序列。BM5d与从Duplan病毒克隆的Du5H基因组非常相似，在gag开放阅读框中具有高度不同的p12序列。在对MAIDS敏感的C57BL / 6小鼠中，感染后2周内在组织中检测到BM5d，但在MAIDS抗性品系A / J感染12周后的组织中未检测到。患有MAIDS的小鼠的B细胞系肿瘤含有并表达BM5d，并且该基因组的克隆整合与感染小鼠中B细胞的克隆扩增存在不同程度的关联。最后，与BM5d探针交叉杂交的mRNA存在于未感染的B6小鼠的脾脏而非肾细胞中。
