Hilger C, Velhagen I, Zentgraf H, Schröder C H
Institut für Virusforschung/ATV, Deutsches Krebsforschungszentrum, Heidelberg, Germany.
J Virol. 1991 Aug;65(8):4284-91. doi: 10.1128/JVI.65.8.4284-4291.1991.
RNA was isolated from tissue of two patients with hepatocellular carcinoma developed on the background of a chronic hepatitis B virus infection. For identification and characterization of 3' ends of X gene open reading frame (ORF)-related transcripts, RNA was reverse transcribed into cDNA and subjected to polymerase chain reaction. Cloned amplification products from tumor tissue of one patient represented an approximately even distribution of transcripts terminating at the established poly(A) signal (standard transcripts) and of truncated transcripts terminating at a CATAAA poly(A) signal within the 3' end region of X gene ORF (truncated transcripts). Amplified cDNA from tumor tissue of the second patient could be attributed mainly to the standard type of transcripts, whereas cDNA from the nontumor tissue of the same patient could be assigned to four groups of transcripts: (i) standard transcripts, (ii) transcripts with internal deletions affecting the 3' end of the X gene, (iii) truncated transcripts, and (iv) hybrid transcripts displaying 5' sequences from the X gene ORF fused to cellular sequences.
从两名在慢性乙型肝炎病毒感染背景下发生肝细胞癌的患者的组织中分离出RNA。为了鉴定和表征X基因开放阅读框（ORF）相关转录本的3'末端，将RNA逆转录成cDNA并进行聚合酶链反应。来自一名患者肿瘤组织的克隆扩增产物代表了在既定的聚腺苷酸化（poly(A)）信号处终止的转录本（标准转录本）和在X基因ORF的3'末端区域内的CATAAA poly(A)信号处终止的截短转录本（截短转录本）的大致均匀分布。来自第二名患者肿瘤组织的扩增cDNA主要可归因于标准类型的转录本，而来自同一名患者非肿瘤组织的cDNA可分为四组转录本：（i）标准转录本，（ii）具有影响X基因3'末端的内部缺失的转录本，（iii）截短转录本，以及（iv）显示来自X基因ORF的5'序列与细胞序列融合的杂合转录本。
