Toiyama Yuji, Araki Toshimitsu, Yoshiyama Shigeyuki, Hiro Jun-ichiro, Miki Chikao, Kusunoki Masato
Second Department of Surgery, Mie University School of Medicine, 2-174 Edobashi, Tsu, Mie, 514-8507, Japan.
Surg Today. 2006;36(3):287-90. doi: 10.1007/s00595-005-3144-y.
The aim of this study was to evaluate the expression pattern of Toll-like receptors (TLRs) in the pouch mucosa of ulcerative colitis patients in comparison with that in the ileum mucosa of noninflammatory bowel disease patients. Pouch mucosal biopsy specimens were collected from postoperative patients who had undergone surgery for ulcerative colitis. Normal ileum specimens were collected from colon cancer patients. The specimens were assessed by immunofluorescence histochemistry using TLR2, TLR3, TLR4, and TLR5 polyclonal antibodies. The normal ileal mucosa constitutively expressed TLR3 and TLR5, whereas TLR2 and TLR4 were barely detectable. In the mucosa of active pouchitis, TLR2 and TLR4 was strongly upregulated, and TLR4 was upregulated even in a noninflamed pouch. No TLR3 or TLR5 expression was detectable. These data suggest that pouchitis may be associated with distinctive changes in selective TLR expression in the pouch mucosa, and that TLR4 alterations in the innate response system may contribute to the pathogenesis of these disorders in particular.
本研究的目的是评估溃疡性结肠炎患者袋状黏膜中Toll样受体（TLRs）的表达模式，并与非炎症性肠病患者的回肠黏膜进行比较。从接受溃疡性结肠炎手术的术后患者中收集袋状黏膜活检标本。从结肠癌患者中收集正常回肠标本。使用TLR2、TLR3、TLR4和TLR5多克隆抗体通过免疫荧光组织化学对标本进行评估。正常回肠黏膜组成性表达TLR3和TLR5，而TLR2和TLR4几乎检测不到。在活动性袋炎的黏膜中，TLR2和TLR4强烈上调，即使在无炎症的袋中TLR4也上调。未检测到TLR3或TLR5表达。这些数据表明，袋炎可能与袋状黏膜中选择性TLR表达的独特变化有关，并且先天反应系统中的TLR4改变可能尤其有助于这些疾病的发病机制。
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