Blankenship R E, Babcock G T, Sauer K
Biochim Biophys Acta. 1975 Apr 14;387(1):165-75. doi: 10.1016/0005-2728(75)90061-4.
Tris-washed chloroplasts which have lost the ability to evolve oxygen can be reactivated by the procedure of Yamashita T., Tsuji, J. and Tomita G. (1971) Plant Cell Physiol. 12, 117-126) [7] to give 100 percent of the rate of control chloroplasts in continuous illumination. Furthermore, in flashing light the reactivated chloroplasts exhibit oxygen-yield oscillations of period four that are characteristic of the control. Similar kinetic parameters for intermediate steps in the water-splitting process are observed for the two preparations. We conclude that the reactivation procedure restores the native oxygen evolution mechanism to Tris-washed chloroplasts. A relatively rapid and reversible (0.5 s decay) light-induced component of EPR Signal II is observed upon inhibition of O2 evolution by Tris washing (Babcock G. T. and Sauer, K. (1975) Biochim. Biophys. Acta 376, 315-328) [10]. Reactivated chloroplasts are similar to untreated chloroplasts in that this Signal IItransient is not observed. Manganese, which is released by Tris treatment to the interior of the thylakoid membrane in an EPR-detectable state, is returned to an EPR-undetectable state by reactivation. The reactivation procedure does not require light to restore O2 evolution and EDTA has no effect on the extent of reactivation. These results are discussed in terms of possible mechanisms for manganese incorporation into photosynthetic membranes.
已丧失放氧能力的经三羟甲基氨基甲烷（Tris）洗涤的叶绿体，可通过山下T.、辻J.和富田G.（1971年，《植物细胞生理学》12卷，117 - 126页）[7]的方法重新激活，在连续光照下达到对照叶绿体速率的100%。此外，在闪光条件下，重新激活的叶绿体呈现出周期为四的放氧振荡，这是对照叶绿体的特征。对于这两种制剂，在水裂解过程中间步骤观察到类似的动力学参数。我们得出结论，重新激活程序将天然放氧机制恢复到经Tris洗涤的叶绿体中。在用Tris洗涤抑制O₂释放时，观察到EPR信号II有一个相对快速且可逆（0.5秒衰减）的光诱导成分（巴布科克G.T.和绍尔K.（1975年），《生物化学与生物物理学报》376卷，315 - 328页）[10]。重新激活的叶绿体与未处理的叶绿体相似，未观察到这种信号II瞬变。经Tris处理以EPR可检测状态释放到类囊体膜内部的锰，通过重新激活恢复到EPR不可检测状态。重新激活程序恢复O₂释放不需要光照，且乙二胺四乙酸（EDTA）对重新激活程度没有影响。根据锰掺入光合膜的可能机制对这些结果进行了讨论。
