Institut für Allgemelne Botanik der Universität Mainz, Saarstr. 21, D-6500, Mainz, Federal Republic of Germany.
Photosynth Res. 1981 Jun;2(2):105-14. doi: 10.1007/BF00028751.
The manganese content of thylakoids and tissues was measured in leaves grown under high- and low-light conditions. Especially when grown in a nutrient medium enriched in manganese (20 μM), the thylakoids contained large amounts of manganese, which could be removed by EDTA washing without impairment of the Hill reaction. The unremovable content of manganese was almost the same in thylakoids from plants grown in nutrient media of normal (2 μM) and reduced (0.2 μM) manganese content. Up to this limit of manganese content, Hill activity did not seem to be impaired. 1.2 atoms Mn per 100 molecules chlorophyll were found in low-light thylakoids and 1.6 atoms Mn in high-light thylakoids. This is similar to the behaviour of other electron transport components, the number of which is also decreased under low-light conditions. However, the decrease in the manganese content is not as striking as the decrease in, for example, the cytochrome f and ferredoxin content. This may be attributed to an invariable pool of manganese which is not involved in the oxygen evolving system. Alternatively, if all of our measured manganese is involved in electron transport to PS II, this could indicate that in low-light chloroplasts the ratio of PS II/PS I components may be somewhat increased.
在高光和低光条件下生长的叶片中测量了类囊体和组织中的锰含量。特别是在富含锰（20 μM）的营养培养基中生长时，类囊体中含有大量的锰，可以通过 EDTA 洗涤除去，而不会损害希尔反应。在正常（2 μM）和低（0.2 μM）锰含量的营养培养基中生长的植物类囊体中，不可去除的锰含量几乎相同。在锰含量达到这个限度之前，希尔活性似乎没有受到损害。在低光下的类囊体中发现每 100 个叶绿素分子中有 1.2 个锰原子，在高光下的类囊体中有 1.6 个锰原子。这与其他电子传递成分的行为相似，其数量在低光条件下也减少了。然而，锰含量的减少并不像细胞色素 f 和铁氧还蛋白含量的减少那样引人注目。这可能归因于不参与产氧系统的锰不变池。或者，如果我们测量的所有锰都参与 PS II 的电子传递，这可能表明在低光下叶绿体中 PS II/PSI 成分的比例可能会略有增加。
