Sharma O K, Roberts W K, Beezley D N, Borek E
Biochim Biophys Acta. 1975 May 16;390(3):327-31. doi: 10.1016/0005-2787(75)90353-6.
An homologous cell-free system from Ehrlich ascites cells was made tRNA dependent by passing both the ribosomes and the ammonium sulfate fraction through DEAE-cellulose columns in 0.30 M KCl. Protein synthesis was routinely stimulated 20-50 fold by the addition of 100 mug/ml of ascites cell tRNA in the presence of oviduct mRNA, ascites mRNA, or encephalomyocarditis RNA.
通过在0.30M KCl中使核糖体和硫酸铵组分都通过DEAE - 纤维素柱，制备了来自艾氏腹水细胞的同源无细胞系统，使其依赖于tRNA。在输卵管mRNA、腹水mRNA或脑心肌炎RNA存在的情况下，通过添加100μg/ml的腹水细胞tRNA，蛋白质合成通常可被刺激20 - 50倍。
