脑心肌炎病毒导致的细胞蛋白质合成关闭：未感染和感染埃氏腹水瘤细胞提取物中病毒和非病毒信使核糖核酸的翻译
Cellular protein synthesis shutoff by mengovirus: translation of nonviral and viral mRNA's in extracts from uninfected and infected Ehrlich ascites tumor cells.
作者信息
Abreu S L, Lucas-Lenard J
出版信息
J Virol. 1976 Apr;18(1):182-94. doi: 10.1128/JVI.18.1.182-194.1976.
Abstract
The mechanism whereby picornaviruses inhibit host protein synthesis while their own synthetic processes proceed unabated has remained elusive. One of our approaches to this problem was to study the ability of cell-free extracts derived from uninfected and mengovirus-infected Ehrlich ascites tumor cells to translate viral and nonviral mRNA's under various conditions of incubation. Our results indicate that viral messengers (from mengovirus and encephalomyocarditis virus) and cellular messengers [L cell and Ehrlich ascites tumor poly(A)-containing mRNA's, rabbit globin mRNA, and chicken embryo lens crystallin mRNA] are translated equally well in both extracts. We also examined the simultaneous translation of viral and nonviral mRNA's in extracts from uninfected Ehrlich ascites tumor cells. Our results indicate that under certain conditions mengovirus RNA can suppress completely the translation of globin mRNA. The significance of these results in terms of the shutoff of host protein synthesis is discussed.
摘要
小核糖核酸病毒在其自身合成过程不受影响的情况下抑制宿主蛋白质合成的机制一直难以捉摸。我们解决这个问题的方法之一是研究从未感染和感染脑心肌炎病毒的艾氏腹水瘤细胞中提取的无细胞提取物在各种孵育条件下翻译病毒和非病毒信使核糖核酸（mRNA）的能力。我们的结果表明，病毒信使（来自脑心肌炎病毒和脑心肌炎病毒）和细胞信使[L细胞和艾氏腹水瘤含多聚腺苷酸（poly(A)）的mRNA、兔珠蛋白mRNA和鸡胚晶状体结晶蛋白mRNA]在两种提取物中翻译效果相同。我们还研究了未感染的艾氏腹水瘤细胞提取物中病毒和非病毒mRNA的同时翻译。我们的结果表明，在某些条件下，脑心肌炎病毒RNA可以完全抑制珠蛋白mRNA的翻译。本文讨论了这些结果在宿主蛋白质合成关闭方面的意义。
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Cellular protein synthesis shutoff by mengovirus: translation of nonviral and viral mRNA's in extracts from uninfected and infected Ehrlich ascites tumor cells.脑心肌炎病毒导致的细胞蛋白质合成关闭：未感染和感染埃氏腹水瘤细胞提取物中病毒和非病毒信使核糖核酸的翻译
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