大鼠腹水肝癌细胞的伴刀豆球蛋白A和蓖麻凝集素介导凝集反应的定量测定。伴刀豆球蛋白A结合与细胞凝集之间的关系。
A quantitative assay for concanavalin A- and Ricinus communis agglutinin-mediated agglutinations of rat ascites hepatoma cells. Relationship between concanavalin A binding and cell agglutination.
作者信息
Kaneko I, Hayatsu H, Ukita T
出版信息
Biochim Biophys Acta. 1975 May 5;392(1):131-40. doi: 10.1016/0304-4165(75)90173-7.
A simple quantitative assay method was developed for the agglutination of rat ascites hepatoma cells mediated by Concanavalin A or Ricinus communis agglutinin. This method was based on the principle that the turbidity of a cell suspension is proportional to the sum of the cross-sectional area of cells and aggregatesmas predicted by the theoretical consideration, the turbidity decreased when cells were aggregated and the decrease was a function of the average number of the cells in aggregates. The agglutinability of the cells, judged by this method, showed a maximum value at a certain concentration of the agglutinin. By further addition of the agglutinin, the agglutinability slightly decreased from the maximum. These phenomena were observed both for Concanavalin A and Ricinus communis agglutinin. The binding and the agglutination experiments using [3-H]concanavalin A revealed that the binding to approx;0% of the total receptors caused a maximal agglutination. This suggested that the receptors responsible for the agglutination constitute only a small part of the total receptors on the surface.
开发了一种简单的定量测定方法，用于检测伴刀豆球蛋白A或蓖麻凝集素介导的大鼠腹水肝癌细胞凝集。该方法基于这样的原理：细胞悬液的浊度与细胞和聚集体的横截面积之和成正比，正如理论考虑所预测的那样，细胞聚集时浊度降低，且降低程度是聚集体中细胞平均数量的函数。用这种方法判断，细胞的凝集性在凝集素的某一浓度下显示出最大值。进一步添加凝集素后，凝集性从最大值略有下降。伴刀豆球蛋白A和蓖麻凝集素均观察到这些现象。使用[3-H]伴刀豆球蛋白A进行的结合和凝集实验表明，与约0%的总受体结合会导致最大凝集。这表明负责凝集的受体仅占表面总受体的一小部分。
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