Macaluso Alessia, Petrinca Annarita, Lanni Luigi, Saccares Stefano, Amiti Sonia, Gabrieli Rosanna, Divizia Maurizio
Zooprofilattico Institute of Latium and Tuscany Regions, Rome, Italy.
J Food Prot. 2006 Feb;69(2):449-52. doi: 10.4315/0362-028x-69.2.449.
In Italy in 1998, hepatitis A virus (HAV) was responsible for an infectious disease transmitted by contaminated bivalve molluscs. To determine the presence of HAV in the bivalves collected during a 1-year follow-up study, hepatitis A RNA was extracted and amplified by a nested reverse transcriptase-PCR method overlapping the VP1/2A region. The HAV genome was detected in 24 (14.1%) of 170 samples: 19 clams (Tapes decussates and Tapes semidecussatus), 1 oyster (Crossostea gigas), and 4 mussels (Mytillus galloprovincialis). Eleven positive samples were collected from marketing areas, and 13 positive samples were collected from growing areas. Seventeen of the 24 positive samples had been taken from domestic products, and 7 had been imported. Sequence analysis showed the presence of genotypes IA and IB. Our results suggest significant presence of HAV in bivalves from both marketing (public consumption) and environmental (growing) areas.
1998年在意大利，甲型肝炎病毒（HAV）引发了一种由受污染双壳贝类传播的传染病。为确定在一项为期1年的随访研究中所采集双壳贝类中是否存在HAV，通过巢式逆转录聚合酶链反应（RT-PCR）方法提取并扩增了甲型肝炎核糖核酸（RNA），该方法覆盖了VP1/2A区域。在170个样本中的24个（14.1%）检测到了HAV基因组：19个蛤（波纹巴非蛤和半纹巴非蛤）、1个牡蛎（长巨牡蛎）和4个贻贝（地中海贻贝）。11个阳性样本采自销售区，13个阳性样本采自养殖区。24个阳性样本中有17个取自国内产品，7个为进口产品。序列分析显示存在IA和IB基因型。我们的结果表明，在来自销售（供公众消费）和环境（养殖）区域的双壳贝类中均大量存在HAV。
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