用于卵胞浆内单精子注射（ICSI）的人类精子中核空泡的存在是否会影响妊娠结局？

Does the presence of nuclear vacuoles in human sperm selected for ICSI affect pregnancy outcome?

作者信息

Berkovitz Arie, Eltes Fina, Ellenbogen Adrian, Peer Sigal, Feldberg Dov, Bartoov Benjamin

机构信息

IVF Unit, Department of Obstetrics and Gynaecology, Sapir Medical Center, Meir Hospital, Kfar Saba, and Male Fertility Laboratory, Faculty of Life Sciences, Bar-Ilan University, Ramat Gan, Israel.

出版信息

Hum Reprod. 2006 Jul;21(7):1787-90. doi: 10.1093/humrep/del049. Epub 2006 Feb 23.

DOI:10.1093/humrep/del049

PMID:16497697

Abstract

BACKGROUND

To verify whether or not microinjection of sperm with a normal nuclear shape but large vacuoles affects IVF-ICSI pregnancy outcome.

METHODS

A comparative study testing IVF outcome parameters of IVF-ICSI, based on morphological selection of spermatozoa with normal nuclei against those based on microinjection of sperm with a normal nuclear shape but large vacuoles. An experimental group, including 28 IVF-ICSI cycles, where only embryos obtained from microinjection of spermatozoa with a normal nuclear shape but large vacuoles were transferred, was matched with a control group, including 28 IVF-ICSI cycles, where only embryos obtained from microinjection of spermatozoa with a strictly defined morphologically normal nuclear shape and content were transferred. The main outcome was IVF-ICSI pregnancy rate.

RESULTS

The experimental group exhibited a significantly lower pregnancy rate per cycle and significantly higher abortion rate per pregnancy compared to the control group (18 versus 50%, and 80 versus 7%, respectively, P=0.01).

CONCLUSION

Microinjection of vacuolated sperm appears to reduce the pregnancy rate and appears to be associated with early abortion.

摘要

背景

验证对具有正常核形态但存在大液泡的精子进行显微注射是否会影响体外受精-卵胞浆内单精子注射（IVF-ICSI）的妊娠结局。

方法

一项对比研究，测试IVF-ICSI的体外受精结局参数，基于对具有正常细胞核的精子进行形态学选择，与基于对具有正常核形态但存在大液泡的精子进行显微注射的情况进行对比。一个实验组，包括28个IVF-ICSI周期，其中仅移植从对具有正常核形态但存在大液泡的精子进行显微注射所获得的胚胎，与一个对照组相匹配，该对照组包括28个IVF-ICSI周期，其中仅移植从对具有严格定义的形态学正常核形态和内容的精子进行显微注射所获得的胚胎。主要结局指标为IVF-ICSI妊娠率。