[子宫颈腺癌和腺鳞癌中HPV DNA检测的原位杂交研究]
[In situ hybridization study on the detection of HPV DNA in adenocarcinoma and adenosquamous carcinoma of the uterine cervix].
作者信息
Fujimoto H, Nagai N, Kioka H, Ohta S, Tanimoto H, Fujiwara A
机构信息
Department of Obstetrics and Gynecology, Hiroshima University School of Medicine.
出版信息
Nihon Sanka Fujinka Gakkai Zasshi. 1991 Jun;43(6):589-94.
Human papillomavirus (HPV) types 16 and 18 have been found closely associated with squamous cell carcinoma and related lesions of the uterine cervix. In order to investigate the relationship between HPV and adenocarcinoma and adenosquamous carcinoma of the uterine cervix, formalin-fixed, paraffinembedded tissues prepared from 38 cases consisting of 30 cases of adenocarcinoma and 8 cases of adenosquamous carcinoma were examined for the presence of HPV DNA by in situ hybridization with digoxigenin labeled HPV 6/11, 16, 18 DNA probes. HPV DNA was localized on the nuclei of the cancer cells in adenocarcinoma and adenosquamous carcinoma. HPV DNA was detected in 13 cases (43.3%) of adenocarcinoma and 4 cases (50.0%) of adenosquamous carcinoma, and HPV type 18 DNA was detected in 13 cases (34.2%) of adenocarcinoma and adenosquamous carcinoma. These findings suggest an association between HPV, especially HPV type 18, and adenocarcinoma and adenosquamous carcinoma of the uterine cervix.
已发现16型和18型人乳头瘤病毒（HPV）与子宫颈鳞状细胞癌及相关病变密切相关。为了研究HPV与子宫颈腺癌及腺鳞癌之间的关系，采用地高辛标记的HPV 6/11、16、18 DNA探针进行原位杂交，对38例由30例腺癌和8例腺鳞癌组成的福尔马林固定、石蜡包埋组织进行HPV DNA检测。HPV DNA定位于腺癌和腺鳞癌癌细胞的细胞核上。在13例（43.3%）腺癌和4例（50.0%）腺鳞癌中检测到HPV DNA，在13例（34.2%）腺癌和腺鳞癌中检测到18型HPV DNA。这些发现提示HPV，尤其是18型HPV，与子宫颈腺癌和腺鳞癌之间存在关联。
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