Fukushima M, Okagaki T, Twiggs L B, Clark B A, Zachow K R, Ostrow R S, Faras A J
Cancer Res. 1985 Jul;45(7):3252-5.
Using the Southern DNA hybridization technique, tissues from 17 cases of invasive carcinoma of the uterine cervix, including nine cases of squamous cell carcinoma, four cases of adenocarcinoma, one case of adenosquamous carcinoma, and three cases of undifferentiated carcinoma, were examined for the presence of human papillomavirus (HPV) DNA. None of the studied cases had histologically confirmed association of condyloma acuminatum or cervical intraepithelial neoplasia in the vicinity. HPV DNA was detected in two of 17 cases under low stringency conditions. One lesion was undifferentiated carcinoma, and another was squamous cell carcinoma. Hybridization under high stringency conditions with a variety of HPV DNA probes indicated the presence of HPV-16 in these two lesions. The other HPV-positive lesion was adenocarcinoma, demonstrating weak hybridizations with HPV-2 and HPV-16 DNA probes only under high stringency conditions. Altogether, three of 17 cases (17.6%) contained HPV DNA. This observation contrasts to the rate of HPV DNA present in 15 of 18 cases (83.3%) of the tissues of cervical intraepithelial neoplasia. Our data suggest that HPV was not consistently detected in invasive squamous cell carcinoma, despite the frequent association of HPV with its supposed precursor lesions of cervical intraepithelial neoplasia.
采用Southern DNA杂交技术，对17例子宫颈浸润癌组织进行检测，其中包括9例鳞状细胞癌、4例腺癌、1例腺鳞癌和3例未分化癌，以检测人乳头瘤病毒（HPV）DNA的存在。所研究的病例在组织学上均未证实附近有尖锐湿 疣或宫颈上皮内瘤变。在低严谨度条件下，17例中有2例检测到HPV DNA。1例病变为未分化癌，另1例为鳞状细胞癌。用多种HPV DNA探针在高严谨度条件下杂交表明这两个病变中存在HPV-16。另一个HPV阳性病变是腺癌，仅在高严谨度条件下与HPV-2和HPV-16 DNA探针有弱杂交。总共，17例中有3例（17.6%）含有HPV DNA。这一观察结果与18例宫颈上皮内瘤变组织中有15例（83.3%）存在HPV DNA的比例形成对比。我们的数据表明，尽管HPV与其假定的宫颈上皮内瘤变前体病变频繁相关，但在浸润性鳞状细胞癌中并非总能检测到HPV。
