Carrier S P, Boulanger P
Can J Comp Med. 1975 Apr;39(2):231-3.
In this study, improvements were made in the technique and the preparation of the antigen. It was possible to perform three extractions and elutions resulting in a soluble reactive preparation from each batch of infected mouse brain. This led to an appreciable increase in the yield of highly reactive antigen. The presence of bluetongue antibodies was not detected in 13,210 sheep sera. Of the 13,486 bovine sera tested, only three questionable reactions were obtained. It was possible to determine that two of these animals were imported. Various isolation methods, including transmission trials to susceptible sheep followed by serological tests on the sheep sera, failed to confirm the infection in the three reactors.
在本研究中，对抗原的技术和制备方法进行了改进。每批感染小鼠脑均能进行三次提取和洗脱，从而得到可溶性反应性制剂。这使得高反应性抗原的产量显著增加。在13210份绵羊血清中未检测到蓝舌病抗体。在检测的13486份牛血清中，仅获得了三个可疑反应。可以确定其中两只动物是进口的。包括将病毒接种到易感绵羊上并随后对绵羊血清进行血清学检测在内的各种分离方法，均未能证实这三只反应阳性动物感染了病毒。
