Ashani Y, Rothschild N, Segall Y, Levanon D, Raveh L
Israel Institute for Biological Research, Kiriat-Shmona.
Life Sci. 1991;49(5):367-74. doi: 10.1016/0024-3205(91)90444-g.
Parathion hydrolase purified from Pseudomonas sp. was injected i.v. into mice to demonstrate the feasibility of using organophosphorus acid anhydride (OPA) hydrolases as pretreatment against organophosphates (OP) poisoning. Results show that exogenous administration of as low as 7 to 26 micrograms of parathion hydrolase conferred protection against challenge with multiple median lethal doses (LD50) of diethyl p-nitrophenyl phosphate (paraoxon; 3.8-7.3 x LD50) and diethylfluorophosphate (DEFP; 2.9 x LD50) without administration of supportive drugs. The extent of protection observed was consistent with blood-parathion hydrolase levels and the kinetic constants of the enzymatic hydrolysis of paraoxon and DEFP by parathion hydrolase. OPA hydrolases not only appear to be potential prophylactic drugs capable of increasing survival ratio following OP intoxication but also to alleviate post-exposure symptoms.
从假单胞菌属中纯化得到的对硫磷水解酶通过静脉注射给小鼠，以证明使用有机磷酸酐（OPA）水解酶作为有机磷（OP）中毒预处理的可行性。结果表明，外源性给予低至7至26微克的对硫磷水解酶，在不给予支持性药物的情况下，可使小鼠免受多倍半数致死剂量（LD50）的对氧磷（3.8 - 7.3×LD50）和二乙基亚磷酸酯（DEFP；2.9×LD50）攻击。观察到的保护程度与血液中对硫磷水解酶水平以及对硫磷水解酶对氧磷和DEFP的酶促水解动力学常数一致。OPA水解酶不仅似乎是能够提高OP中毒后存活率的潜在预防性药物，而且还能减轻接触后的症状。
