一种测定血清γ-谷氨酰转肽酶的临床方法。
A clinical method for the determination of serum gamma-glutamyl transpeptidase.
作者信息
Tamaoki H, Minato S, Takei S, Fujisawa K
出版信息
Clin Chim Acta. 1975 Nov 15;65(1):21-7. doi: 10.1016/0009-8981(75)90330-7.
PMID:165
Abstract
A simple, highly sensitive and reproducible method for the assay of gamma-glutamyl transpeptidase (EC 2.3.2-) activity is introduced, using gamma-glutamyl-p-nitroanilide as a substrate and glycylglycine as an acceptor in 50 g/l of polyoxyethylene nonylphenol. Serum transpeptidase activity was assayed in 1080 healthy adults, the normal mean value being 14.8 mU/ml. The diagnostic evaluation of the enzyme in various hepatobiliary diseases is also discussed.
摘要
介绍了一种简单、高度灵敏且可重复的γ-谷氨酰转肽酶（EC 2.3.2-）活性测定方法，该方法以γ-谷氨酰对硝基苯胺为底物，甘氨酰甘氨酸为受体，在50 g/l的聚氧乙烯壬基酚中进行。对1080名健康成年人的血清转肽酶活性进行了测定，正常平均值为14.8 mU/ml。还讨论了该酶在各种肝胆疾病中的诊断评估。
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