Brorson Ø, Brorson S-H
Department of Microbiology, Vestfold Sentralsykehus, Tønsberg, Norway.
Infection. 2006 Feb;34(1):26-8. doi: 10.1007/s15010-006-4121-0.
In this study the new ketolide telithromycin was tested in vitro against motile and cystic forms of Borrelia afzelii, one of the species of Borrelia burgdorferi sensu lato. Acridine orange staining, dark field microscopy, and transmission electron microscopy were the techniques used to study the influence of telithromycin on the bacteria. The activity was unexpectedly high, 0.0003 microg/ml < MBC < or = 0.0006 microg/ml for the mobile forms after 7 days of incubation at 34 degrees C. MIC was < 0.00015 microg/ml. It is likely that the agent works bacteriostatically and kills in a time-dependent and concentration-independent way, by binding tightly to the ribosomes. The agent was not able to prevent cyst formation, and the cysts were not affect ed at an in vivo achievable concentration. Electron microscopy also supports the hypothesis of telithromycin being an effective agent against the mobile bacteria.
在本研究中，新型酮内酯类药物泰利霉素针对狭义伯氏疏螺旋体中的阿氏疏螺旋体的游动型和包囊型进行了体外试验。采用吖啶橙染色、暗视野显微镜检查和透射电子显微镜检查等技术来研究泰利霉素对细菌的影响。其活性出乎意料地高，在34℃孵育7天后，游动型的最低杀菌浓度（MBC）为0.0003微克/毫升＜MBC≤0.0006微克/毫升。最低抑菌浓度（MIC）＜0.00015微克/毫升。该药物可能通过与核糖体紧密结合发挥抑菌作用，并以时间依赖性和浓度非依赖性方式杀菌。该药物无法阻止包囊形成，且在体内可达到的浓度下包囊不受影响。电子显微镜检查也支持泰利霉素是一种针对游动细菌的有效药物这一假说。
