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基于从废弃鱼类副产品中获得的蛋白胨，以具有成本效益的海洋培养基生产海洋益生菌。

Production of Marine Probiotic Bacteria in a Cost-Effective Marine Media Based on Peptones Obtained from Discarded Fish By-Products.

作者信息

Vázquez José Antonio, Durán Ana, Nogueira Margarita, Menduíña Araceli, Antunes Joana, Freitas Ana Cristina, Gomes Ana María

机构信息

Grupo de Biotecnología y Bioprocesos Marinos, Instituto de Investigaciones Marinas (IIM-CSIC), C/Eduardo Cabello, 6, CP 36208 Vigo, Galicia, Spain.

Laboratorio de Reciclado y Valorización de Materiales Residuales (REVAL), Instituto de Investigaciones Marinas (IIM-CSIC), C/Eduardo Cabello, 6, CP 36208 Vigo, Galicia, Spain.

出版信息

Microorganisms. 2020 Jul 26;8(8):1121. doi: 10.3390/microorganisms8081121.

DOI:10.3390/microorganisms8081121
PMID:32722528
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7464406/
Abstract

The industrial production of marine bacteria with probiotic properties is limited by the excessive cost of the culture media adequate for their growth. The present work aimed to study the suitability of 30 marine media formulated with nitrogen sources (fish peptones) from different fish discards and seawater, for the growth of two marine probiotic bacteria (MPB), namely sp. and . These fish peptones were produced from several discarded fish and by-products (heads, skins, and whole individuals of megrim, mackerel, gurnard, hake, etc.). In all cultivations, no significant differences were found on cell viability when the microorganism grew on commercial or alternative media. In relation to the biomass production, the growth of sp. on waste media was commonly similar or a 20% lower than observed in the control cultures. For , various peptones (skin peptones of pouting and blue whiting) showed even higher productive ability than commercial peptones. An initial economical evaluation revealed that low-cost media reduced until 120 times the cost of production of MPB.

摘要

具有益生菌特性的海洋细菌的工业化生产受到适合其生长的培养基成本过高的限制。目前的工作旨在研究用来自不同鱼类废弃物的氮源（鱼蛋白胨）和海水配制的30种海洋培养基对两种海洋益生菌（MPB），即sp.和 的生长适宜性。这些鱼蛋白胨由几种废弃鱼类和副产物（鲽鱼头、皮以及鲐鱼、鲂鮄、无须鳕等整鱼）制成。在所有培养中，当微生物在商业培养基或替代培养基上生长时，未发现细胞活力有显著差异。关于生物量生产，sp.在废弃培养基上的生长通常与对照培养中观察到的相似或低20%。对于 ，各种蛋白胨（长蛇鲻和蓝鳕的皮蛋白胨）显示出比商业蛋白胨更高的生产能力。初步经济评估表明，低成本培养基将MPB的生产成本降低了120倍。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b55/7464406/41a380f67ec2/microorganisms-08-01121-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b55/7464406/5156cea6b790/microorganisms-08-01121-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b55/7464406/c650595c263c/microorganisms-08-01121-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b55/7464406/41a380f67ec2/microorganisms-08-01121-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b55/7464406/5156cea6b790/microorganisms-08-01121-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b55/7464406/c650595c263c/microorganisms-08-01121-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8b55/7464406/41a380f67ec2/microorganisms-08-01121-g003.jpg

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