Rodríguez-Bustamante E, Maldonado-Robledo G, Sánchez-Contreras A, Klimova T, Arreguín-Espinosa R, Sánchez S
Departamento de Biología Molecular y Biotecnología del Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México, DF 04510, Mexico.
Appl Microbiol Biotechnol. 2006 Jul;71(4):568-73. doi: 10.1007/s00253-006-0330-9. Epub 2006 Feb 25.
In this work, we report on the synthesis and ability of the mesoporous material MCM-41 to adsorb the norisoprenoid beta-ionone. This compound, with a violet aroma note, can be produced from lutein by the yeast Trichosporon asahii through a bioconversion process. We found that beta-ionone inhibited the yeast growth and constrained aroma formation. Growth inhibition was overcome using silicate MCM-41 as sorbent device in a fermentation system that allowed product removal from the culture medium by headspace manipulation. Compared to a commercial silica gel, the mesoporous material exhibited a 4.5-fold higher beta-ionone adsorption. Contrasting to cultures without the sorbent device, the presence of MCM-41 allowed a marked increase (14-fold) in beta-ionone production. Our results suggested that confinement of the norisoprenoid into the sorbent material bypassed its toxicity which allowed a better beta-ionone production. This study represents the first report on the use of MCM-41 to recover an aroma produced by fermentation and therefore, a novel application for a mesoporous material.
在本研究中，我们报道了介孔材料MCM - 41吸附降异戊二烯类化合物β-紫罗兰酮的合成及性能。这种具有紫罗兰香气的化合物可由酵母阿萨希毛孢子菌通过生物转化过程从叶黄素中产生。我们发现β-紫罗兰酮会抑制酵母生长并限制香气形成。在一个通过顶空操作从培养基中去除产物的发酵系统中，使用硅酸盐MCM - 41作为吸附装置克服了生长抑制。与商用硅胶相比，这种介孔材料对β-紫罗兰酮的吸附能力高出4.5倍。与没有吸附装置的培养物相比，MCM - 41的存在使β-紫罗兰酮的产量显著增加（14倍）。我们的结果表明，将降异戊二烯类化合物限制在吸附材料中可规避其毒性，从而实现更好的β-紫罗兰酮产量。本研究是关于使用MCM - 41回收发酵产生的香气的首次报道，因此，是介孔材料的一种新应用。
