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霍氏肠杆菌将叶黄素生物转化形成一种新化合物8-甲基-α-紫罗兰酮。

Bioconversion of lutein by Enterobacter hormaechei to form a new compound, 8-methyl-α-ionone.

作者信息

Zhong Guifang, Wang Fangfang, Sun Jianhong, Ye Jianbin, Mao Duobin, Ma Ke, Yang Xuepeng

机构信息

School of Food and Biological Engineering, Zhengzhou University of Light Industry, Zhengzhou, 450002, China.

China Tobacco, Shandong Industrial Co. Ltd., Jinan, 250014, China.

出版信息

Biotechnol Lett. 2017 Jul;39(7):1019-1024. doi: 10.1007/s10529-017-2329-y. Epub 2017 Mar 28.

DOI:10.1007/s10529-017-2329-y
PMID:28353147
Abstract

OBJECTIVES

To investigate the final product of the bioconversion of lutein by a novel lutein-degrading bacterium, Enterobacter hormaechei A20, and the kinetics of the process.

RESULTS

A new product, 8-methyl-α-ionone, was resolved by GC-MS. The compound was further identified by NMR. A conversion yield of 90% was achieved by E. hormaechei in 36 h with 10 g lutein l.

CONCLUSIONS

This is the first report of the bioconversion of lutein to form 8-methyl-α-ionone. A degradation pathway is proposed.

摘要

目的

研究新型叶黄素降解细菌霍氏肠杆菌A20对叶黄素生物转化的最终产物及其过程动力学。

结果

通过气相色谱-质谱联用仪解析出一种新产物8-甲基-α-紫罗兰酮。该化合物通过核磁共振进一步鉴定。霍氏肠杆菌在36小时内利用10克/升叶黄素实现了90%的转化率。

结论

这是关于叶黄素生物转化形成8-甲基-α-紫罗兰酮的首次报道。提出了一条降解途径。

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