Maldonado-Robledo G, Rodriguez-Bustamante E, Sanchez-Contreras A, Rodriguez-Sanoja R, Sanchez S
Departamento de Biología Molecular y Biotecnología del Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México., México, DF 04510, México.
Appl Microbiol Biotechnol. 2003 Oct;62(5-6):484-8. doi: 10.1007/s00253-003-1315-6. Epub 2003 Jun 24.
A mixed culture formed by Bacillus sp. and Geotrichum sp. produced tobacco aroma compounds from the carotenoid lutein through the formation of the intermediate beta-ionone. Both microorganisms can grow independently in a medium supplemented with lutein, but only Geotrichum produces beta-ionone. This intermediate was incorporated by the bacilli, converted to aroma and this product excreted to the culture medium. Bacillus sp. did not utilize beta-ionone for growth but modified it. We conclude that, in the bioconversion of lutein to products with tobacco aroma, Geotrichum sp. is involved in carotenoid oxidation to produce beta-ionone and Bacillus sp. is responsible for the norisoprenoid reduction to produce 7,8-dihydro-beta-ionone and 7,8-dihydro-beta-ionol.
芽孢杆菌属和地霉属形成的混合培养物通过中间产物β-紫罗兰酮的形成，从类胡萝卜素叶黄素产生烟草香气化合物。两种微生物都能在添加叶黄素的培养基中独立生长，但只有地霉属产生β-紫罗兰酮。这种中间产物被芽孢杆菌吸收，转化为香气并将该产物分泌到培养基中。芽孢杆菌属不利用β-紫罗兰酮进行生长但会对其进行修饰。我们得出结论，在叶黄素生物转化为具有烟草香气的产物过程中，地霉属参与类胡萝卜素氧化以产生β-紫罗兰酮，而芽孢杆菌属负责去甲类异戊二烯还原以产生7,8-二氢-β-紫罗兰酮和7,8-二氢-β-紫罗兰醇。
