Tang Dianping, Yuan Ruo, Chai Yaqin, Zhong Xia, Liu Yan, Dai Jianyuan
Key Laboratory of Analytical Chemistry, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P.R. China.
Clin Biochem. 2006 Mar;39(3):309-14. doi: 10.1016/j.clinbiochem.2005.12.003. Epub 2006 Feb 28.
A novel potentiometric immunosensor for the detection of hepatitis B surface antigen has been developed by self-assembling gold nanoparticles to a thiol-containing sol-gel network.
A cleaned gold electrode was first immersed in a hydrolyzed (3-mercaptopropyl) trimethoxysilane sol-gel solution to assemble a three-dimensional silica gel, and then gold nanoparticles were absorbed onto the thiol groups of the sol-gel network. Finally, hepatitis B surface antibody was assembled onto the surface of the gold nanoparticles. The self-assembling procedure was characterized by cyclic voltammetry and electrochemical impedance spectroscopy. Detection is based on the change in potentiometric response before and after the antigen-antibody reaction.
Tests relating to the detection of hepatitis B surface antigen demonstrate that the potentiometric immunosensor exhibited a rapid potentiometric response (<4 min), with high sensitivity, good reproducibility, and long-term stability. The linear range was from 4 to 960 ng.mL(-1) with a detection limit of 1.9 ng.mL(-1) (S/N = 3) and the lifetime was 1 month.
Analytical results of several specimens using the developed technique showed satisfactory agreement with those from an ELISA method. This method shows promise for detecting HBsAg in clinical specimens.
通过将金纳米颗粒自组装到含硫醇的溶胶 - 凝胶网络中，开发了一种用于检测乙型肝炎表面抗原的新型电位免疫传感器。
首先将清洁后的金电极浸入水解的（3 - 巯基丙基）三甲氧基硅烷溶胶 - 凝胶溶液中以组装三维硅胶，然后将金纳米颗粒吸附到溶胶 - 凝胶网络的硫醇基团上。最后，将乙型肝炎表面抗体组装到金纳米颗粒表面。通过循环伏安法和电化学阻抗谱对自组装过程进行表征。检测基于抗原 - 抗体反应前后电位响应的变化。
与乙型肝炎表面抗原检测相关的测试表明，该电位免疫传感器呈现出快速的电位响应（<4分钟），具有高灵敏度、良好的重现性和长期稳定性。线性范围为4至960 ng.mL(-1)，检测限为1.9 ng.mL(-1)（S/N = 3），使用寿命为1个月。
使用所开发技术对多个样本的分析结果与酶联免疫吸附测定法的结果显示出令人满意的一致性。该方法在检测临床样本中的乙肝表面抗原方面具有前景。
