伊朗人、绵羊和骆驼体内细粒棘球绦虫分离株的特性分析。
Characterization of Echinococcus granulosus isolates from human, sheep and camel in Iran.
作者信息
Ahmadi N, Dalimi A
机构信息
Parasitology and Mycology Department, Shahid Beheshti University of Medical Sciences, P.O. Box 19395-4618, Tehran, IR Iran.
出版信息
Infect Genet Evol. 2006 Mar;6(2):85-90. doi: 10.1016/j.meegid.2005.01.005. Epub 2005 Mar 2.
In the present study, Echinoccocus granulosus isolates collected from human, sheep and camel samples in Iran were characterized based on rostellar hook morphology of protoscoleces as well as PCR-RFLP. Morphological study on human and animal isolates showed the presence of two distinct strains of the parasite, one in sheep and the other one in camels. In this regard, rostellar hook of sheep isolates were significantly different from those of camel origin, meanwhile human isolates were found to be similar to those isolated from sheep. Molecular analysis of the ITS1 region of rDNA derived from human, sheep and camel isolates were in agreement with the morphological findings. Based on the PCR-RFLP method, the sheep and human isolates appeared to pertain to the same genotype and the camel isolates were appeared to pertain to a different genotype.
在本研究中，基于原头节的顶突钩形态以及聚合酶链反应-限制性片段长度多态性（PCR-RFLP）对从伊朗的人类、绵羊和骆驼样本中收集的细粒棘球绦虫分离株进行了特征分析。对人类和动物分离株的形态学研究表明存在两种不同的寄生虫菌株，一种存在于绵羊中，另一种存在于骆驼中。在这方面，绵羊分离株的顶突钩与骆驼来源的顶突钩有显著差异，同时发现人类分离株与从绵羊中分离出的分离株相似。对来自人类、绵羊和骆驼分离株的核糖体DNA（rDNA）的内转录间隔区1（ITS1）进行的分子分析与形态学研究结果一致。基于PCR-RFLP方法，绵羊和人类分离株似乎属于同一基因型，而骆驼分离株似乎属于不同的基因型。
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