Eroglu Fadime, Dokur Mehmet, Ulu Yüksel
Department of Parasitology, Faculty of Medicine, Aksaray University, Aksaray, Turkey.
FaBiyosit Microbiology-Biotechnology R&D Co., Adana, Turkey.
Iran J Parasitol. 2021 Apr-Jun;16(2):327-335. doi: 10.18502/ijpa.v16i2.6284.
The migration of humans and climatic and environmental changes cause the emergence of infectious diseases. This study aimed to investigate the changes in the molecular epidemiology of the echinococcosis in the southeast region of Turkey after migrations.
Overall, 159 tissues samples were taken from suspected cases of echinococcosis at the Kilis State Hospital in the southeast region of Turkey. All of the tissues samples were analyzed using histopathology methods, PCR, Real-time PCR methods, DNA sequencing, and phylogenetic analyses in laboratories.
The positivity values of the histopathology, the polymerase chain reaction, and the Real-time PCR methods were found to be 14.5% (23/159),15.7% (25/159), and 16.9% (27/159), respectively. 32.0 % (8/25) of isolates and 68% (17/25) of isolates were identified using PCR methods. 58.8% (10/17) of the isolates were found to be Genotype 1% and 41.2% (7/17) isolates were found to be Genotype 3.
Molecular methods play an important role in the epidemiology, treatment, and diagnosis of diseases. Increasing immigration in a geographical area may create social, economic, and health problems in that area. For this reason, epidemiological studies of infectious diseases should be updated in areas with immigration.
人类迁徙以及气候和环境变化导致传染病的出现。本研究旨在调查土耳其东南部地区在人口迁徙后棘球蚴病分子流行病学的变化。
总体而言，从土耳其东南部地区基利斯州立医院的棘球蚴病疑似病例中采集了159份组织样本。所有组织样本均在实验室中采用组织病理学方法、聚合酶链反应（PCR）、实时荧光定量PCR方法、DNA测序和系统发育分析进行分析。
组织病理学、聚合酶链反应和实时荧光定量PCR方法的阳性率分别为14.5%（23/159）、15.7%（25/159）和16.9%（27/159）。采用PCR方法鉴定出32.0%（8/25）的分离株和68%（17/25）的分离株。发现58.8%（10/17）的分离株为基因型1，41.2%（7/17）的分离株为基因型3。
分子方法在疾病的流行病学、治疗和诊断中发挥着重要作用。一个地理区域内移民的增加可能会给该地区带来社会、经济和健康问题。因此，在有移民的地区应更新传染病的流行病学研究。
