Department of Medical Parasitology & Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Asian Pac J Trop Med. 2012 Jan;5(1):58-61. doi: 10.1016/S1995-7645(11)60246-0.
To find importance of morphometric criterion of larval rostellar hook of Echinococcus granulosus (E. granulosus) and the easy and reliable method for distinguish sheep and camel strains in epidemiologic studies.
Larval rostellar hooks (n=1860) of 31 camel and sheep isolates in Iran, which already had been characterized by PCR, were carefully processed by computerized imagime analysis system (CIAS) and acquired data about rostellar hooks were analyzed using software SPSS.
Measurement analysis of rostellar hooks [mean length (24.23±3.12) μ m] indicated that length of the large hook was a remarkable parameter for strain differentiation. Data analysis demonstrated that CIAS could be used as a reliable tool to distinguish camel from sheep strains with high sensitivity (95.2%) and specificity (91.5%).
CIAS as a specific, sensitive, economic, fast, and reliable means might be used for differentiation of E. granulosus strains. Although perimeter and area were measured by digital technology, they were not shown as discriminative criterion as total hook length did.
在流行病学研究中,寻找细粒棘球蚴(E. granulosus)幼虫头节钩形态计量学标准的重要性,以及区分绵羊和骆驼株的简单可靠方法。
对来自伊朗的 31 株绵羊和骆驼分离株的 1860 个幼虫头节钩进行仔细处理,使用计算机图像分析系统(CIAS)进行处理,并使用 SPSS 软件分析头节钩的获取数据。
头节钩的测量分析[平均长度(24.23±3.12)μm]表明,大钩的长度是菌株分化的一个显著参数。数据分析表明,CIAS 可以作为一种可靠的工具,用于区分绵羊株和骆驼株,具有高灵敏度(95.2%)和特异性(91.5%)。
CIAS 作为一种特异、敏感、经济、快速和可靠的手段,可用于区分棘球蚴株。虽然周长和面积是通过数字技术测量的,但它们并没有像总钩长那样作为区分标准。