Xie Wei, Bao Yong, Yu Li-jian, Hou Guang-nan, Tan Hong-xiang
Department of Internal Medicine, School of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2006 Feb;26(2):177-80.
To study the effect of saikosaponins, the active ingredients of Bupleurum chinense DC, on epileptic seizure and EEG of pentetrazole (PTZ)-induced chronic kindling rats.
Forty-eight healthy Sprague-Dawley rats were randomly divided into 6 equal groups, namely the blank control group, normal saline (NS) group, sodium valproate (VPA) group, and 3 saikosaponins groups of high, medium and small doses. Except those in the blank control group, the rats in the other groups were all given different treatments as specified prior to intraperitoneal PTZ injection to induce kindling on a daily basis for 4 consecutive weeks. Epileptic seizures of the rats were recorded during the treatment and EEG recorded at the end of the treatments.
Seizure frequency in the 3 saikosaponins groups decreased 2 weeks later, which was especially obvious in the high-dose group (P<0.05). The kindling rate was significantly lower in high-dose saikosaponins group than in the other treatment groups after 4 weeks of the treatment (P<0.05), with also less intense seizure onset (P<0.01) and differences in the wave form of EEG.
Saikosaponins can inhibit PTZ-induced epileptic seizure in kindling rats and antagonize the kindling effect of PTZ.
研究柴胡有效成分柴胡皂苷对戊四氮（PTZ）诱导的慢性点燃大鼠癫痫发作及脑电图的影响。
将48只健康的Sprague-Dawley大鼠随机分为6组，每组8只，即空白对照组、生理盐水（NS）组、丙戊酸钠（VPA）组以及柴胡皂苷高、中、低剂量组。除空白对照组外，其余各组大鼠在腹腔注射PTZ前均给予不同处理，每日1次，连续4周诱导点燃。治疗期间记录大鼠癫痫发作情况，治疗结束时记录脑电图。
柴胡皂苷3个剂量组大鼠癫痫发作频率在2周后均降低，高剂量组尤为明显（P<0.05）。治疗4周后，柴胡皂苷高剂量组点燃率显著低于其他治疗组（P<0.05），癫痫发作起始强度也较弱（P<0.01），脑电图波形也有差异。
柴胡皂苷可抑制PTZ诱导的点燃大鼠癫痫发作，拮抗PTZ的点燃作用。
