Xie Wei, Li Chang-zheng, Bao Yong, Yu Li-jian
Department of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2006 Aug;26(8):1132-5.
To study the effect of saikosaponins, the active ingredients of Bupleurum chinense DC. on glutamate and GABA expressions in the hippocampus of slow kindling rats induced by pentetrazole.
Forty-eight healthy Sprague-Dawley rats were randomly divided into 6 equal groups, namely the blank control group (group A), normal saline (NS) group (group B), sodium valproate group (group C), and 3 saikosaponins groups of high, medium and small doses (groups D, E, and F, respectively). The rats in each group other than group A were given corresponding treatments after slow kindling by pentetrazole. After 4 weeks of treatment, the rats were sacrifices and the brain tissues were sampled, sliced and stained by immunohistochemically, and the results were analyzed according to the positive cell number and gray scale.
In CA1 region, the glutamate-positive cell number and gray scale of group B was significantly different from the other groups (P<0.05), but such difference was not observed in the CA2 and DG (P>0.05); In CA1, CA2 and DG of the hippocampus, the GABA-positive cell number of group B was significantly greater but the gray scale lower than those of the other groups (P<0.05). In CA1 and CA2 regions of the hippocampus, the glutamate- and GABA-positive cell ratio of group B was significantly lower than that of the other groups (P<0.05), but in CA1, CA2, and DG region of the hippocampus, the ratio of gray scale between glutamate- and GABA-positive cells was comparable between the groups (P>0.05).
The expression of glutamate and GABA, especially the latter, increased in chronic kindling rat hippocampus. Saikosaponins intervene in such changes of glutamate and GABA to contain their expressions within normal range, which may be one of the mechanisms of saikosaponins to inhibit slow kindling induced by pentetrazole.
研究柴胡的活性成分柴胡皂苷对戊四氮诱导的慢点燃大鼠海马中谷氨酸和γ-氨基丁酸（GABA）表达的影响。
将48只健康的Sprague-Dawley大鼠随机分为6组，每组8只，分别为空白对照组（A组）、生理盐水（NS）组（B组）、丙戊酸钠组（C组）以及柴胡皂苷高、中、低剂量组（分别为D组、E组和F组）。除A组外，其余各组大鼠经戊四氮慢点燃后给予相应处理。治疗4周后，处死大鼠，取脑组织进行取材、切片，采用免疫组织化学法染色，根据阳性细胞数和灰度值进行结果分析。
在海马CA1区，B组谷氨酸阳性细胞数及灰度值与其他各组相比差异有统计学意义（P<0.05），但在CA2区和齿状回（DG）未观察到这种差异（P>0.05）；在海马CA1、CA2和DG区，B组GABA阳性细胞数显著多于其他各组，但灰度值低于其他各组（P<0.05）。在海马CA1和CA2区，B组谷氨酸和GABA阳性细胞比例显著低于其他各组（P<0.05），但在海马CA1、CA2和DG区，谷氨酸和GABA阳性细胞之间的灰度值比例在各组间相当（P>0.05）。
慢性点燃大鼠海马中谷氨酸和GABA的表达增加，尤其是后者。柴胡皂苷干预谷氨酸和GABA的这种变化，使其表达维持在正常范围内，这可能是柴胡皂苷抑制戊四氮诱导的慢点燃的机制之一。
