[在线血液透析滤过置换液中细菌内毒素的动态比浊法鲎试剂检测]

[Kinetic turbidimetric limulus assay of bacterial endotoxin in the replacement solution of on-line hemodiafiltration].

作者信息

Lao Hai-yan, Lin Qiu-xiao, Yang Min, Liang Xin-ling, Shi Wei, Li Hui, Feng Ju-jin

机构信息

Department of Pharmacy, People's Hospital of Guangdong Province, Guangzhou 510080, China.

出版信息

Nan Fang Yi Ke Da Xue Xue Bao. 2006 Feb;26(2):242-4.

PMID:16503544

Abstract

OBJECTIVE

To determine bacterial endotoxin in the replacement solution of on-line hemodiafiltration (on-line HDF) using kinetic turbidimetric limulus test.

METHODS AND RESULTS

Validation test was performed with the replacement solution of on-line HDF in which quantified standard endotoxin was added. The recovery rates of endotoxin from the replacement solution and its dilutions at 1/5, 1/10, and 1/20 were 58.17%, 106.7%, 99.00% and 98.79%, respectively, suggesting that the optimal dilution was at 1/10. Standard endotoxin was added into the replacement solution of on-line HDF of 3 batches (040408, 040511,040527), and the recovery rates in their dilution at 1/10 were 76.32%, 99.00% and 96.24%, respectively. The standard endotoxin in the working curve was 1.00, 0.125, and 0.0156 Eu/ml (endotoxin unit/ml), and the dilution at 1/10 of the replacement solution is effective to eliminate the interference in limulus test.

CONCLUSION

Kinetic turbidimetric limulus test provide a means to detect endotoxin in the replacement solution of on-line HDF.

摘要

目的

采用动态浊度法鲎试剂检测法测定在线血液透析滤过（on-line HDF）置换液中的细菌内毒素。

方法与结果

对添加了定量标准内毒素的在线HDF置换液进行验证试验。置换液及其1/5、1/10和1/20稀释液中内毒素的回收率分别为58.17%、106.7%、99.00%和98.79%，表明最佳稀释倍数为1/10。向3批（040408、040511、040527）在线HDF置换液中添加标准内毒素，其1/10稀释液的回收率分别为76.32%、99.00%和96.24%。工作曲线中的标准内毒素为1.00、0.125和0.0156 Eu/ml（内毒素单位/毫升），置换液1/10稀释液可有效消除鲎试剂检测中的干扰。