Soloff M S, Rees H D, Sar M, Stumpf W E
Endocrinology. 1975 Jun;96(6):1475-7. doi: 10.1210/endo-96-6-1475.
[3-H]Oxytocin was incubated in vitro with pieces of oviduct and mammary gland and the tissue were subjected to autoradiography. Radioactivity was localized only in smooth muscle cells of the oviduct and in regions of mammary tissue where myoepithelial cells are found. In contrast, radioactivity was not concentrated in any region of skeletal muscle, a nontarget tissue for oxytocin. The localization of radioactivity in oviduct and mammary cells was absent when the tissues were incubated with [3-H]oxytocin and an excess of unlabeled oxytocin. These findings provide evidence for the presence of specific and high affinity receptors for oxytocin in its target tissues.
将[3-H]催产素与输卵管和乳腺组织块在体外进行孵育，然后对这些组织进行放射自显影。放射性仅定位在输卵管的平滑肌细胞以及发现肌上皮细胞的乳腺组织区域。相比之下，放射性未集中在骨骼肌的任何区域，骨骼肌是催产素的非靶组织。当组织与[3-H]催产素和过量未标记的催产素一起孵育时，输卵管和乳腺细胞中的放射性定位消失。这些发现为催产素在其靶组织中存在特异性高亲和力受体提供了证据。
