空气/水界面处的传输是蛋白质微阵列中出现环状物的原因。

Transport at the air/water interface is the reason for rings in protein microarrays.

作者信息

Deng Yang, Zhu X-Y, Kienlen Taryn, Guo Athena

机构信息

Department of Chemistry, University of Minnesota, Minneapolis, MN 55455, USA.

出版信息

J Am Chem Soc. 2006 Mar 8;128(9):2768-9. doi: 10.1021/ja057669w.

DOI:10.1021/ja057669w

PMID:16506733

Abstract

Fabricating a protein microarray involves the deposition of nanoliter droplets of solutions on a solid surface. Instead of uniform spots, one often observes ring-like structures that add to the difficulty in quantification. We show that the accumulation of proteins at the air/water interface of the nanodroplet is the reason. Transformation to a uniform spot can be achieved via the addition of competitive surfactants or the control of surface reaction kinetics.

摘要

制造蛋白质微阵列涉及将纳升溶液液滴沉积在固体表面上。人们常常观察到的不是均匀的斑点，而是环状结构，这增加了定量的难度。我们表明，蛋白质在纳米液滴的空气/水界面处的积累就是原因。通过添加竞争性表面活性剂或控制表面反应动力学可以实现向均匀斑点的转变。