Holbach L M, Font R L, Baehr W, Pittler S J
Department of Ophthalmology, Cullen Eye Institute, Baylor College of Medicine, Houston, TX 77030.
Curr Eye Res. 1991;10 Suppl:63-8. doi: 10.3109/02713689109020359.
Fifty-one corneal buttons obtained by penetrating keratoplasty from patients with a preoperative clinical diagnosis of nonulcerative herpetic keratitis and/or disciform stromal scarring (44) as well as ulcerative necrotizing stromal keratitis (7) were processed for herpes simplex virus (HSV) antigens using an immunoperoxidase technique and for HSV DNA by the polymerase chain reaction (PCR). HSV antigens were detected significantly more often (p less than 0.025) in specimens with avascular nonulcerative keratitis than in those with vascularization. In contrast to HSV antigens, HSV DNA was identified at equal proportions in avascular and vascularized lesions. Both HSV antigens and HSV DNA were detected in all specimens from patients with ulcerative necrotizing stromal keratitis. The implications of these findings with regard to possible mechanisms underlying herpetic keratitis in man are discussed.
对51个通过穿透性角膜移植术获取的角膜植片进行处理，这些患者术前临床诊断为非溃疡性疱疹性角膜炎和/或盘状基质瘢痕形成（44例）以及溃疡性坏死性基质角膜炎（7例）。采用免疫过氧化物酶技术检测单纯疱疹病毒（HSV）抗原，并用聚合酶链反应（PCR）检测HSV DNA。在无血管性非溃疡性角膜炎标本中检测到HSV抗原的频率显著高于（p<0.025）有血管化的标本。与HSV抗原相反，在无血管和有血管化病变中HSV DNA的检出比例相同。在溃疡性坏死性基质角膜炎患者的所有标本中均检测到HSV抗原和HSV DNA。讨论了这些发现对人类疱疹性角膜炎潜在机制的意义。
