• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Crystallization and preliminary crystallographic analysis of the nickel-responsive regulator NikR from Pyrococcus horikoshii.嗜热栖热菌镍响应调节蛋白NikR的结晶及初步晶体学分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt 1):43-5. doi: 10.1107/S1744309104025473. Epub 2004 Oct 16.
2
Structure of Pyrococcus horikoshii NikR: nickel sensing and implications for the regulation of DNA recognition.嗜热栖热菌镍响应调控蛋白（NikR）的结构：镍感知及其对DNA识别调控的影响
J Mol Biol. 2005 May 6;348(3):597-607. doi: 10.1016/j.jmb.2005.03.017.
3
Apo and nickel-bound forms of the Pyrococcus horikoshii species of the metalloregulatory protein: NikR characterized by molecular dynamics simulations.热球菌属金属调节蛋白 apo 和镍结合形式：NikR 的分子动力学模拟特性。
Biochemistry. 2009 Dec 22;48(50):12024-33. doi: 10.1021/bi9013352.
4
Purification, crystallization and preliminary crystallographic analysis of the vacuole-type ATPase subunit E from Pyrococcus horikoshii OT3.嗜热栖热菌OT3液泡型ATP酶亚基E的纯化、结晶及初步晶体学分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt 1):56-8. doi: 10.1107/S1744309104026430. Epub 2004 Oct 23.
5
Crystallization and preliminary X-ray analysis of PH1010 from Pyrococcus horikoshii OT3, a member of the archaeal DUF54 family of proteins.嗜热栖热菌OT3来源的PH1010（古菌DUF54蛋白家族成员）的结晶及初步X射线分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jun 1;63(Pt 6):532-4. doi: 10.1107/S1744309107024487. Epub 2007 May 31.
6
Expression, purification, crystallization and preliminary X-ray diffraction analysis of galactokinase from Pyrococcus horikoshii.嗜热栖热菌半乳糖激酶的表达、纯化、结晶及初步X射线衍射分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Feb 1;62(Pt 2):169-71. doi: 10.1107/S1744309106001813. Epub 2006 Jan 27.
7
Crystallization and preliminary X-ray analysis of PH1566, a putative ribosomal RNA-processing factor from the hyperthermophilic archaeon Pyrococcus horikoshii OT3.嗜热古菌火球菌OT3中一种假定的核糖体RNA加工因子PH1566的结晶及初步X射线分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Jan 1;62(Pt 1):47-8. doi: 10.1107/S1744309105040613. Epub 2005 Dec 16.
8
The MinD protein from the hyperthermophilic archaeon Pyrococcus horikoshii: crystallization and preliminary X-ray analysis.来自嗜热古菌火球菌的MinD蛋白：结晶及初步X射线分析。
Acta Crystallogr D Biol Crystallogr. 2001 Jun;57(Pt 6):896-7. doi: 10.1107/s0907444901005728. Epub 2001 May 25.
9
Purification, crystallization and preliminary X-ray crystallographic analysis of the archaeal phosphoglycerate mutase PH0037 from Pyrococcus horikoshii OT3.来自嗜热栖热菌OT3的古菌磷酸甘油酸变位酶PH0037的纯化、结晶及初步X射线晶体学分析。
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Aug 1;62(Pt 8):788-90. doi: 10.1107/S1744309106026121. Epub 2006 Jul 25.
10
Preparation, crystallization and preliminary X-ray diffraction analysis of PH1948, predicted RNA methyltransferase from Pyrococcus horikoshii.来自嗜热栖热菌的预测RNA甲基转移酶PH1948的制备、结晶及初步X射线衍射分析
J Zhejiang Univ Sci B. 2005 Jun;6(6):454-6. doi: 10.1631/jzus.2005.B0454.

引用本文的文献

1
Physical basis of metal-binding specificity in Escherichia coli NikR.大肠杆菌NikR中金属结合特异性的物理基础
J Am Chem Soc. 2009 Jul 29;131(29):10220-8. doi: 10.1021/ja9026314.

本文引用的文献

1
Processing of X-ray diffraction data collected in oscillation mode.振荡模式下收集的X射线衍射数据的处理。
Methods Enzymol. 1997;276:307-26. doi: 10.1016/S0076-6879(97)76066-X.
2
Screening and optimization strategies for macromolecular crystal growth.大分子晶体生长的筛选与优化策略
Acta Crystallogr D Biol Crystallogr. 1994 Jul 1;50(Pt 4):414-23. doi: 10.1107/S0907444994002660.
3
Structure and implications for the thermal stability of phosphopantetheine adenylyltransferase from Thermus thermophilus.嗜热栖热菌中磷酸泛酰巯基乙胺腺苷酰转移酶的结构及其对热稳定性的影响
Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):97-104. doi: 10.1107/s0907444903025319. Epub 2003 Dec 18.
4
Crystal structure of the nickel-responsive transcription factor NikR.镍响应转录因子NikR的晶体结构。
Nat Struct Biol. 2003 Oct;10(10):794-9. doi: 10.1038/nsb985. Epub 2003 Sep 14.
5
Nickel coordination is regulated by the DNA-bound state of NikR.镍的配位由镍反应调控蛋白（NikR）的DNA结合状态所调节。
Nat Struct Biol. 2003 Feb;10(2):126-30. doi: 10.1038/nsb890.
6
NikR repressor: high-affinity nickel binding to the C-terminal domain regulates binding to operator DNA.NikR阻遏蛋白：镍与C端结构域的高亲和力结合调节其与操纵基因DNA的结合。
Chem Biol. 2002 Oct;9(10):1141-8. doi: 10.1016/s1074-5521(02)00241-7.
7
Regulation of high affinity nickel uptake in bacteria. Ni2+-Dependent interaction of NikR with wild-type and mutant operator sites.细菌中高亲和力镍摄取的调控。NikR 与野生型和突变型操纵子位点的 Ni2+ 依赖性相互作用。
J Biol Chem. 2000 Jun 30;275(26):19735-41. doi: 10.1074/jbc.M002232200.
8
Nickel transport systems in microorganisms.微生物中的镍转运系统。
Arch Microbiol. 2000 Jan;173(1):1-9. doi: 10.1007/s002030050001.
9
NikR is a ribbon-helix-helix DNA-binding protein.NikR是一种带状-螺旋-螺旋DNA结合蛋白。
Protein Sci. 1999 Nov;8(11):2494-500. doi: 10.1110/ps.8.11.2494.
10
Structure/function relationships in nickel metallobiochemistry.镍金属生物化学中的结构/功能关系
Curr Opin Chem Biol. 1999 Apr;3(2):188-99. doi: 10.1016/S1367-5931(99)80032-5.

嗜热栖热菌镍响应调节蛋白NikR的结晶及初步晶体学分析

Crystallization and preliminary crystallographic analysis of the nickel-responsive regulator NikR from Pyrococcus horikoshii.

作者信息

Kitao Tomoe, Kuroishi Chizu, Tahirov Tahir H

机构信息

Highthroughput Factory, RIKEN Harima Institute, 1-1-1 Kouto, Hyogo 679-5148, Japan.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Jan 1;61(Pt 1):43-5. doi: 10.1107/S1744309104025473. Epub 2004 Oct 16.

DOI:10.1107/S1744309104025473
PMID:16508086
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1952384/
Abstract

The nickel-responsive repressor from Pyrococcus horikoshii OT3 (PhNikR) has been crystallized in the apo form (PhNikR-apo) and two nickel-bound forms (PhNikR-Ni-1 and PhNikR-Ni-2). The PhNikR-apo crystals belong to space group P2(1), with unit-cell parameters a = 75.78, b = 54.32, c = 77.28 A, beta = 116.07 degrees, and diffract to 2.2 A. The PhNikR-Ni-1 crystals belong to space group P4(1)2(1)2, with unit-cell parameters a = b = 99.89, c = 97.98 A, and diffract to 3.0 A and the PhNikR-Ni-2 crystals belong to space group P3(1)21 or P3(2)21, with unit-cell parameters a = b = 109.95, c = 79.0 A, and diffract to 2.1 A. The crystals obtained were suitable for detailed structural studies.

摘要

来自嗜热栖热菌OT3（PhNikR）的镍响应阻遏蛋白已被结晶为无辅基形式（PhNikR-apo）和两种镍结合形式（PhNikR-Ni-1和PhNikR-Ni-2）。PhNikR-apo晶体属于空间群P2(1)，晶胞参数为a = 75.78，b = 54.32，c = 77.28 Å，β = 116.07°，衍射分辨率为2.2 Å。PhNikR-Ni-1晶体属于空间群P4(1)2(1)2，晶胞参数为a = b = 99.89，c = 97.98 Å，衍射分辨率为3.0 Å；PhNikR-Ni-2晶体属于空间群P3(1)21或P3(2)21，晶胞参数为a = b = 109.95，c = 79.0 Å，衍射分辨率为2.1 Å。所获得的晶体适合进行详细的结构研究。