Coulthwaite L, Pretty I A, Smith P W, Higham S M, Verran J
Department of Biological Sciences, Manchester Metropolitan University, Manchester, UK.
Caries Res. 2006;40(2):112-6. doi: 10.1159/000091056.
The aim of this study was to determine the microbiological origin of plaque fluorescence observed during quantitative light-induced fluorescence (QLF) analysis. Plaque was sampled from dentures, because of easy accessibility and the homogeneous background provided by the denture tooth during imaging, and the acknowledged comparability to occlusal plaque. Forty removable poly(methyl methacrylate) dentures were screened for the presence of fluorescent plaque deposits during QLF analysis. Dentures were photographed, QLF images were recorded and samples of fluorescent plaque were taken. Plaque samples were cultured on fastidious anaerobe agar, Wilkins Chalgren agar and Sabourauds dextrose agar. Plates were screened under QLF and fluorescent colonies were subcultured and identified. Areas of red, orange and green fluorescence were detected on the fitting and non-fitting surfaces of dentures. The red and orange fluorescing species were Prevotella melaninogenica, Actinomyces israelii and Candida albicans, which are generally acknowledged to be secondary colonisers, present in more mature plaque. Green fluorescence was observed in streptococcal species (early colonisers) and Fusobacterium nucleatum (important organism in plaque development). Non-fluorescent colonies were also cultured. Plaque which accumulates on susceptible surfaces tends to be associated with caries, but it may be its maturity, rather than the presence of cariogenic streptococci, that is more likely to provide a microbiological link between red fluorescence and caries.
本研究的目的是确定在定量光诱导荧光（QLF）分析过程中观察到的菌斑荧光的微生物来源。由于假牙易于获取，且在成像过程中假牙牙齿提供了均匀的背景，同时假牙与咬合面菌斑具有公认的可比性，因此从假牙上采集菌斑。在QLF分析过程中，对40副可摘式聚甲基丙烯酸甲酯假牙进行荧光菌斑沉积物检测。对假牙进行拍照，记录QLF图像，并采集荧光菌斑样本。菌斑样本在苛求厌氧菌琼脂、威尔金斯·查尔格伦琼脂和沙氏葡萄糖琼脂上培养。在QLF下对平板进行筛选，对荧光菌落进行传代培养和鉴定。在假牙的贴合面和非贴合面上检测到红色、橙色和绿色荧光区域。发出红色和橙色荧光的菌种为产黑色素普雷沃菌、以色列放线菌和白色念珠菌，这些通常被认为是在更成熟菌斑中存在的二次定植菌。在链球菌属菌种（早期定植菌）和具核梭杆菌（菌斑发育中的重要微生物）中观察到绿色荧光。也培养出了非荧光菌落。在易感表面积聚的菌斑往往与龋齿有关，但可能是其成熟度，而非致龋性链球菌的存在，更有可能在红色荧光与龋齿之间提供微生物学联系。
