用于溶解在低粘度流体中的包封蛋白核磁共振波谱分析的新型表面活性剂混合物。
Novel surfactant mixtures for NMR spectroscopy of encapsulated proteins dissolved in low-viscosity fluids.
作者信息
Peterson Ronald W, Pometun Maxim S, Shi Zhengshuang, Wand A Joshua
机构信息
Department of Biochemistry and Biophysics, University of Pennsylvania, Philadelphia, PA 19104-6059, USA.
出版信息
Protein Sci. 2005 Nov;14(11):2919-21. doi: 10.1110/ps.051535405. Epub 2005 Sep 30.
Abstract
NMR spectroscopy of encapsulated proteins dissolved in low-viscosity fluids is emerging as a tool for biophysical studies of proteins in atomic detail in a variety of otherwise inaccessible contexts. The central element of the approach is the encapsulation of the protein of interest within the aqueous core of a reverse micelle with high structural fidelity. The process of encapsulation is highly dependent upon the nature of the surfactant(s) employed. Here we describe novel mixtures of surfactants that are capable of successfully encapsulating a range of types of proteins under a variety of conditions.
摘要
溶解于低粘度流体中的包封蛋白质的核磁共振光谱正成为一种工具，用于在各种其他难以实现的情况下对蛋白质进行原子水平的生物物理研究。该方法的核心要素是将目标蛋白质以高结构保真度包封在反胶束的水核内。包封过程高度依赖于所使用表面活性剂的性质。在此，我们描述了新型表面活性剂混合物，它们能够在各种条件下成功包封一系列类型的蛋白质。
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