Lazar Iulia M, Grym Jakub, Foret Frantisek
Virginia Bioinformatics Institute and Department of Biological Sciences, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061, USA.
Mass Spectrom Rev. 2006 Jul-Aug;25(4):573-94. doi: 10.1002/mas.20081.
Instrument miniaturization is one way of addressing the issues of sensitivity, speed, throughput, and cost of analysis in DNA diagnostics, proteomics, and related biotechnology areas. Microfluidics is of special interest for handling very small sample amounts, with minimal concerns related to sample loss and cross-contamination, problems typical for standard fluidic manipulations. Furthermore, the small footprint of these microfabricated structures leads to instrument designs suitable for high-density, parallel sample processing, and high-throughput analyses. In addition to miniaturized systems designed with optical or electrochemical detection, microfluidic devices interfaced to mass spectrometry have also been demonstrated. Instruments for automated sample infusion analysis are now commercially available, and microdevices utilizing chromatographic or capillary electrophoresis separation techniques are under development. This review aims at documenting the technologies and applications of microfluidic mass spectrometry for the analysis of proteomic samples.
仪器小型化是解决DNA诊断、蛋白质组学及相关生物技术领域中灵敏度、速度、通量和分析成本等问题的一种方式。微流控技术对于处理极少量样品特别有意义，与标准流体操作中典型的样品损失和交叉污染问题相关的担忧最小。此外，这些微加工结构的占地面积小，使得仪器设计适合高密度、并行样品处理和高通量分析。除了设计用于光学或电化学检测的小型化系统外，与质谱联用的微流控装置也已得到证实。用于自动样品注入分析的仪器现已商业化，利用色谱或毛细管电泳分离技术的微型装置也正在开发中。本综述旨在记录微流控质谱技术在蛋白质组学样品分析中的技术和应用。
