Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing 100084, China.
Lab Chip. 2013 Sep 7;13(17):3309-22. doi: 10.1039/c3lc50449b. Epub 2013 Jul 3.
Instrument miniaturization is one of the critical issues to improve sensitivity, speed, throughput, and to reduce the cost of analysis. Microfluidics possesses the ability to handle small sample amounts, with minimal concerns related to sample loss and cross-contamination, problems typical for standard fluidic manipulations. Moreover, the native properties of microfluidics provide the potential for high-density, parallel sample processing, and high-throughput analysis. Recently, the coupling of microfluidic devices to mass spectrometry, especially electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI), has attracted an increasing interest and produced tremendous achievements. The interfaces between microfluidics and mass spectrometry are one of the primary focused problems. In this review, we summarize the latest achievements since 2008 in the field of the technologies and applications in the combining of microfluidics with ESI-MS and MALDI-MS. The integration of several analytical functions on a microfluidic device such as sample pretreatment and separations before sample introduction into the mass spectrometer is also discussed.
仪器微型化是提高灵敏度、速度、通量和降低分析成本的关键问题之一。微流控技术具有处理小样本量的能力，最小化了与样品损失和交叉污染相关的问题，这些问题是标准流体操作的典型问题。此外，微流控技术的固有特性为高密度、并行的样品处理和高通量分析提供了潜力。最近，微流控装置与质谱，特别是电喷雾电离（ESI）和基质辅助激光解吸/电离（MALDI）的耦合引起了越来越多的关注，并取得了巨大的成就。微流控与质谱之间的接口是主要关注的问题之一。在本文中，我们总结了自 2008 年以来在微流控与 ESI-MS 和 MALDI-MS 结合领域的技术和应用的最新进展。还讨论了在将样品引入质谱仪之前，在微流控装置上集成多个分析功能，如样品预处理和分离。
