编码核糖核酸酶H的基因rnh的大肠杆菌部分功能突变体的活性与表型的相关性
Correlation of activity with phenotypes of Escherichia coli partial function mutants of rnh, the gene encoding RNase H.
作者信息
Itaya M, Crouch R J
机构信息
Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892.
出版信息
Mol Gen Genet. 1991 Jul;227(3):433-7. doi: 10.1007/BF00273934.
The rnh gene of Escherichia coli encodes RNase H. rnh mutants display at least two phenotypes: (1) they require functional RecBCD enzyme for growth; thus rnh-339::cat recB270 (Ts) and rnh-339::cat recC271 (Ts) strains are temperature sensitive for growth; (2) rnh mutants permit replication that is independent of the chromosomal origin, presumably by failing to remove RNA-DNA hybrids from which extra-original replication can be primed. We report here that manifestation of these two phenotypes occurs at different levels of RNase H function; we have examined partially functional rnh mutants for their in vitro RNase H activity, their ability to rescue viability in recB or recC cells and their ability to permit growth of mutants incapable of using oriC [dnaA (Ts)].
大肠杆菌的rnh基因编码核糖核酸酶H。rnh突变体表现出至少两种表型：（1）它们的生长需要功能性的RecBCD酶；因此，rnh-339::cat recB270（Ts）和rnh-339::cat recC271（Ts）菌株对温度敏感；（2）rnh突变体允许独立于染色体原点的复制，推测是由于未能去除可用于引发额外原点复制的RNA-DNA杂交体。我们在此报告，这两种表型的表现发生在核糖核酸酶H功能的不同水平；我们已经检测了部分功能性的rnh突变体的体外核糖核酸酶H活性、它们在recB或recC细胞中拯救生存能力的能力以及它们允许无法使用oriC的突变体生长的能力【dnaA（Ts）】。
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