Rinia Hilde A, Bonn Mischa, Müller Michiel
Swammerdam Institute for Life Sciences, University of Amsterdam, P.O. Box 94062, 1090 GB Amsterdam, The Netherlands.
J Phys Chem B. 2006 Mar 9;110(9):4472-9. doi: 10.1021/jp0564849.
A novel procedure is developed to describe and reproduce experimental coherent anti-Stokes Raman scattering (CARS) data, with particular emphasis on highly congested spectral regions. The approach, exemplified here with high-quality multiplex CARS data, makes use of spontaneous Raman scattering results. It is shown that the underlying vibrational Raman response can be retrieved from the multiplex CARS spectra, so that the Raman spectrum can be reconstituted, provided an adequate signal-to-noise ratio (SNR) is present in the experimental data and sufficient a priori knowledge of the vibrational resonances involved exists. The conversion of CARS to Raman data permits a quantitative interpretation of CARS spectra. This novel approach is demonstrated for highly congested multiplex CARS spectra of adenosine mono-, di-, and triphosphate (AMP, ADP, and ATP), nicotinamide adenine dinucleotide (NAD+), and small unilamellar vesicles (SUVs) of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC). Quantitative determination of nucleotide concentrations and composition analysis in mixtures is demonstrated.
开发了一种新方法来描述和重现实验性相干反斯托克斯拉曼散射（CARS）数据，特别强调高度拥挤的光谱区域。这里以高质量的多路复用CARS数据为例的方法利用了自发拉曼散射结果。结果表明，只要实验数据中存在足够的信噪比（SNR）并且对所涉及的振动共振有足够的先验知识，就可以从多路复用CARS光谱中检索出潜在的振动拉曼响应，从而重构拉曼光谱。将CARS转换为拉曼数据可以对CARS光谱进行定量解释。这种新方法在腺苷一磷酸、二磷酸和三磷酸（AMP、ADP和ATP）、烟酰胺腺嘌呤二核苷酸（NAD+）以及1-棕榈酰-2-油酰-sn-甘油-3-磷酸胆碱（POPC）的小单层囊泡（SUV）的高度拥挤的多路复用CARS光谱中得到了验证。展示了混合物中核苷酸浓度的定量测定和成分分析。
