生物医学中的相干拉曼散射显微镜术
Coherent Raman Scattering Microscopy in Biology and Medicine.
作者信息
Zhang Chi, Zhang Delong, Cheng Ji-Xin
机构信息
Weldon School of Biomedical Engineering and Department of Chemistry, Purdue University, West Lafayette, Indiana 47907; email:
出版信息
Annu Rev Biomed Eng. 2015;17:415-45. doi: 10.1146/annurev-bioeng-071114-040554. Epub 2015 Oct 22.
Abstract
Advancements in coherent Raman scattering (CRS) microscopy have enabled label-free visualization and analysis of functional, endogenous biomolecules in living systems. When compared with spontaneous Raman microscopy, a key advantage of CRS microscopy is the dramatic improvement in imaging speed, which gives rise to real-time vibrational imaging of live biological samples. Using molecular vibrational signatures, recently developed hyperspectral CRS microscopy has improved the readout of chemical information available from CRS images. In this article, we review recent achievements in CRS microscopy, focusing on the theory of the CRS signal-to-noise ratio, imaging speed, technical developments, and applications of CRS imaging in bioscience and clinical settings. In addition, we present possible future directions that the use of this technology may take.
摘要
相干拉曼散射（CRS）显微镜技术的进步使得在活体系统中对功能性内源性生物分子进行无标记可视化和分析成为可能。与自发拉曼显微镜相比，CRS显微镜的一个关键优势是成像速度显著提高，从而实现了对活生物样品的实时振动成像。利用分子振动特征，最近开发的高光谱CRS显微镜改进了从CRS图像中获取化学信息的读出。在本文中，我们回顾了CRS显微镜的最新成果，重点关注CRS信噪比理论、成像速度、技术发展以及CRS成像在生物科学和临床环境中的应用。此外，我们还提出了该技术未来可能的发展方向。
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本文引用的文献
1
Microsecond Scale Vibrational Spectroscopic Imaging by Multiplex Stimulated Raman Scattering Microscopy.基于多重受激拉曼散射显微镜的微秒级振动光谱成像
Light Sci Appl. 2015;4. doi: 10.1038/lsa.2015.38.
2
High-Speed Coherent Raman Fingerprint Imaging of Biological Tissues.生物组织的高速相干拉曼指纹成像
Nat Photonics. 2014;8:627-634. doi: 10.1038/nphoton.2014.145.
3
Assessing cholesterol storage in live cells and C. elegans by stimulated Raman scattering imaging of phenyl-Diyne cholesterol.通过苯基二炔胆固醇的受激拉曼散射成像评估活细胞和秀丽隐杆线虫中的胆固醇储存情况。
Sci Rep. 2015 Jan 22;5:7930. doi: 10.1038/srep07930.
4
Imaging complex protein metabolism in live organisms by stimulated Raman scattering microscopy with isotope labeling.通过同位素标记的受激拉曼散射显微镜对活生物体中的复杂蛋白质代谢进行成像。
ACS Chem Biol. 2015 Mar 20;10(3):901-8. doi: 10.1021/cb500787b. Epub 2015 Jan 15.
5
Stimulated Raman Scattering Microscopy with a Robust Fibre Laser Source.基于强大光纤激光源的受激拉曼散射显微镜
Nat Photonics. 2014 Feb 1;8(2):153-159. doi: 10.1038/nphoton.2013.360.
6
Imaging lipid metabolism in live Caenorhabditis elegans using fingerprint vibrations.利用指纹振动对活线虫的脂质代谢进行成像。
Angew Chem Int Ed Engl. 2014 Oct 27;53(44):11787-92. doi: 10.1002/anie.201406029. Epub 2014 Sep 4.
7
Label-free real-time imaging of myelination in the Xenopus laevis tadpole by in vivo stimulated Raman scattering microscopy.通过体内受激拉曼散射显微镜对非洲爪蟾蝌蚪髓鞘形成进行无标记实时成像。
J Biomed Opt. 2014 Aug;19(8):086005. doi: 10.1117/1.JBO.19.8.086005.
8
Imaging the intracellular distribution of tyrosine kinase inhibitors in living cells with quantitative hyperspectral stimulated Raman scattering.利用定量高光谱受激拉曼散射成像活细胞中酪氨酸激酶抑制剂的细胞内分布。
Nat Chem. 2014 Jul;6(7):614-22. doi: 10.1038/nchem.1961. Epub 2014 May 25.
9
Hyperspectral and differential CARS microscopy for quantitative chemical imaging in human adipocytes.用于人体脂肪细胞定量化学成像的高光谱和差分相干反斯托克斯拉曼散射显微镜术
Biomed Opt Express. 2014 Apr 2;5(5):1378-90. doi: 10.1364/BOE.5.001378. eCollection 2014 May 1.
10
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