Kawai Akito, Higuchi Shigesada, Tsunoda Masaru, Nakamura Kazuo T, Miyamoto Shuichi
Faculty of Pharmaceutical Sciences, Sojo University, 4-22-1 Ikeda, Kumamoto 860-0082, Japan.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Dec 1;65(Pt 12):1282-4. doi: 10.1107/S1744309109044479. Epub 2009 Nov 27.
Crenarchaeal PCNA is known to consist of three subunits (PCNA1, PCNA2 and PCNA3) that form a heterotrimer (PCNA123). Recently, another heterotrimeric PCNA composed of only PCNA2 and PCNA3 was identified in Sulfolobus tokodaii strain 7 (stoPCNAs). In this study, the purified stoPCNA2-stoPCNA3 complex was crystallized by hanging-drop vapour diffusion. The crystals obtained belonged to the orthorhombic space groups I222 and P2(1)2(1)2, with unit-cell parameters a = 91.1, b = 111.8, c = 170.9 A and a = 91.1, b = 160.6, c = 116.6 A, respectively. X-ray diffraction data sets were collected to 2.90 A resolution for the I222 crystals and to 2.80 A resolution for the P2(1)2(1)2 crystals.
已知泉古菌增殖细胞核抗原（PCNA）由三个亚基（PCNA1、PCNA2和PCNA3）组成，它们形成一个异源三聚体（PCNA123）。最近，在嗜热栖热菌7号菌株（stoPCNAs）中鉴定出另一种仅由PCNA2和PCNA3组成的异源三聚体PCNA。在本研究中，通过悬滴气相扩散法使纯化的stoPCNA2-stoPCNA3复合物结晶。获得的晶体属于正交晶系空间群I222和P2(1)2(1)2，其晶胞参数分别为a = 91.1、b = 111.8、c = 170.9 Å和a = 91.1、b = 160.6、c = 116.6 Å。收集到I222晶体的X射线衍射数据集，分辨率为2.90 Å；收集到P2(1)2(1)2晶体的X射线衍射数据集，分辨率为2.80 Å。
