Pérez-Sánchez Ricardo, Ramajo-Hernández Alicia, Ramajo-Martín Vicente, Oleaga Ana
Unidad de Patología Animal, Instituto de Recursos Naturales y Agrobiología de Salamanca (CSIC), Salamanca, Spain.
Proteomics. 2006 Apr;6 Suppl 1:S226-36. doi: 10.1002/pmic.200500420.
Schistosoma bovis is a ruminant pathogen that is poorly known at a molecular level. With an aim of identifying the parasite proteins involved in host-parasite interplay, we studied two protein extracts that contain, respectively, the proteins excreted/secreted by the adult worm (ES) and the tegumental proteins exposed to the host (TG). The 2-DE, 2-D immunoblot and MS were employed to separate and identify the antigenic proteins and the most abundant non-antigenic proteins in each extract. There were some 400 and 600 spots detected in the ES and the TG extracts, respectively. Ninety-six spots were subjected to MS analysis and 64 of them were identified. Overall, we identified 18 S. bovis proteins located at the host-parasite interface, 16 of which have not been identified previously in this parasite, and one of which -lysozyme- has never been reported in a Schistosoma species. Of the proteins identified, at least 4 can counteract host defence mechanisms. The other proteins are also likely to play some role in the host-parasite relationships. Therefore, studies in grater depth on all these proteins will provide a better understanding of how this parasite interacts with its host and new strategies for anti-schistosome drug or vaccine design.
牛血吸虫是一种在分子水平上了解甚少的反刍动物病原体。为了鉴定参与宿主-寄生虫相互作用的寄生虫蛋白，我们研究了两种蛋白质提取物，分别包含成虫排泄/分泌的蛋白质（ES）和暴露于宿主的体表蛋白质（TG）。采用二维电泳（2-DE）、二维免疫印迹和质谱（MS）来分离和鉴定每种提取物中的抗原蛋白和最丰富的非抗原蛋白。在ES提取物和TG提取物中分别检测到约400个和600个斑点。对96个斑点进行了质谱分析，其中64个得到了鉴定。总体而言，我们鉴定出18种位于宿主-寄生虫界面的牛血吸虫蛋白，其中16种在此寄生虫中以前未被鉴定过，其中一种——溶菌酶——从未在血吸虫物种中报道过。在鉴定出的蛋白质中，至少有4种可以对抗宿主防御机制。其他蛋白质也可能在宿主-寄生虫关系中发挥某种作用。因此，对所有这些蛋白质进行更深入的研究将有助于更好地理解这种寄生虫如何与宿主相互作用，并为抗血吸虫药物或疫苗设计提供新策略。
