Ng Lisa Fp, Barr Ian, Nguyen Tung, Noor Suriani Mohd, Tan Rosemary Sok-Pin, Agathe Lora V, Gupta Sanjay, Khalil Hassuzana, To Thanh Long, Hassan Sharifah Syed, Ren Ee-Chee
Genome Institute of Singapore, 60 Biopolis Street, Genome, #02-01, 138672, Singapore.
BMC Infect Dis. 2006 Mar 2;6:40. doi: 10.1186/1471-2334-6-40.
Continuous outbreaks of the highly pathogenic H5N1 avian influenza A in Asia has resulted in an urgent effort to improve current diagnostics to aid containment of the virus and lower the threat of a influenza pandemic. We report here the development of a PCR-based assay that is highly specific for the H5N1 avian influenza A virus.
A one-step reverse-transcription PCR assay was developed to detect the H5N1 avian influenza A virus. The specificity of the assay was shown by testing sub-types of influenza A virus and other viral and bacterial pathogens; and on field samples.
Validation on 145 field specimens from Vietnam and Malaysia showed that the assay was specific without cross reactivity to a number of other infuenza strains as well as human respiratory related pathogens. Detection was 100% from allantoic fluid in H5N1 positive samples, suggesting it to be a reliable sampling source for accurate detection.
The assay developed from this study indicates that the primers are specific for the H5N1 influenza virus. As shown by the field tested results, this assay would be highly useful as a diagnostic tool to help identify and control influenza epidemics.
亚洲高致病性H5N1甲型禽流感的持续爆发促使人们迫切努力改进当前的诊断方法，以协助控制该病毒并降低流感大流行的威胁。我们在此报告一种基于PCR的检测方法的开发，该方法对H5N1甲型禽流感病毒具有高度特异性。
开发了一种一步法逆转录PCR检测方法来检测H5N1甲型禽流感病毒。通过检测甲型流感病毒的亚型以及其他病毒和细菌病原体，并对现场样本进行检测，证明了该检测方法的特异性。
对来自越南和马来西亚的145份现场样本进行的验证表明，该检测方法具有特异性，不会与许多其他流感毒株以及人类呼吸道相关病原体发生交叉反应。H5N1阳性样本的尿囊液检测率为100%，表明它是准确检测的可靠采样来源。
本研究开发的检测方法表明，这些引物对H5N1流感病毒具有特异性。现场测试结果表明，该检测方法作为一种诊断工具，对于帮助识别和控制流感疫情将非常有用。
