恰加斯病性食管炎不同演变阶段细胞因子（MIG、干扰素-γ、肿瘤坏死因子-α、白细胞介素-4、白细胞介素-5和白细胞介素-10）的评估。
Evaluation of cytokines (MIG, IFN-gamma, TNF-alpha, IL-4, IL-5, and IL-10) during the different evolutive phases of chagasic esophagopathy.
作者信息
Crema Eduardo, Monteiro Isabela de Oliveira, Gomes Marília Gabriela Zanier, Silva Alex Augusto, Rodrigues Júnior Virmondes
机构信息
Disciplines of the Digestive Apparatus and Immunology, Federal University of Triângulo Mineiro, Uberaba, MG, Brazil.
出版信息
Clin Immunol. 2006 May;119(2):213-8. doi: 10.1016/j.clim.2005.12.011. Epub 2006 Feb 28.
The production of cytokines (MIG, IFN-gamma, TNF-alpha, IL-4, IL-5, and IL-10) was studied in 39 individuals, including 28 with chagasic esophagopathy and 11 nonchagasic patients with gastroesophageal reflux disease. A sandwich enzyme immunoassay employing monoclonal antibody pairs specific for each cytokine was used. IFN-gamma and MIG production was significantly higher in patients with megaesophagus compared to control. Furthermore, in the absence of stimulation TNF-alpha levels were lower in the chagasic group than in the control group. In addition, significantly lower TNF-alpha levels were observed for the advanced form of the disease compared to the nonadvanced form. These results support the hypothesis that, although patients with advanced phase of megaesophagus present low number of CD4+ T lymphocytes, PBMC from this patients are able to respond up specific antigen stimulation.
对39名个体进行了细胞因子（MIG、干扰素-γ、肿瘤坏死因子-α、白细胞介素-4、白细胞介素-5和白细胞介素-10）产生情况的研究，其中包括28例患有恰加斯病性食管病的患者和11例非恰加斯病性胃食管反流病患者。采用了一种夹心酶免疫测定法，该方法使用针对每种细胞因子的特异性单克隆抗体对。与对照组相比，巨食管患者的干扰素-γ和MIG产生显著更高。此外，在无刺激情况下，恰加斯病组的肿瘤坏死因子-α水平低于对照组。另外，与疾病非进展期相比，疾病进展期的肿瘤坏死因子-α水平显著更低。这些结果支持以下假设：尽管巨食管晚期患者的CD4 + T淋巴细胞数量较少，但该患者的外周血单核细胞能够对特定抗原刺激产生反应。
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