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Insulin regulation of the glucagon gene is mediated by an insulin-responsive DNA element.

作者信息

Philippe J

机构信息

Department of Microbiology, Centre Médical Universitaire, Geneva, Switzerland.

出版信息

Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):7224-7. doi: 10.1073/pnas.88.16.7224.

DOI:10.1073/pnas.88.16.7224
PMID:1651499
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC52266/
Abstract

Diabetes mellitus is characterized by insulin deficiency and high plasma glucagon levels, which can be normalized by insulin replacement. It has previously been reported that glucagon gene expression is negatively regulated by insulin at the transcriptional level. By transfection studies, I have now localized a DNA control element that mediates insulin effects on glucagon gene transcription. This element also confers insulin responsiveness to a heterologous promoter. DNA-binding proteins that specifically interact with this insulin-responsive element are found in both glucagon- and non-glucagon-producing cells; and the pattern of binding, as assessed by the gel retardation assay, is not modified by prior insulin treatment.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e264/52266/b198fd63edb2/pnas01066-0336-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e264/52266/0e927b53f48e/pnas01066-0336-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e264/52266/c4c32c0b4b07/pnas01066-0336-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e264/52266/b198fd63edb2/pnas01066-0336-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e264/52266/0e927b53f48e/pnas01066-0336-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e264/52266/c4c32c0b4b07/pnas01066-0336-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e264/52266/b198fd63edb2/pnas01066-0336-c.jpg

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本文引用的文献

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DNA-dependent transcription of adenovirus genes in a soluble whole-cell extract.腺病毒基因在可溶性全细胞提取物中的DNA依赖性转录。
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