Manoharan S, Kolanjiappan K, Suresh K, Panjamurthy K
Department of Biochemistry, Annamalai University, Annamalai Nagar, India.
Indian J Med Res. 2005 Dec;122(6):529-34.
BACKGROUND & OBJECTIVE: Overproduction of lipid peroxidation byproducts and disturbances in antioxidant defense system have been implicated in the pathogenesis of several diseases including oral cancer. Though several studies have been done on the level of lipid peroxidation and antioxidants in oral cancer patients, there are no reports in patients with various clinical stages of oral squamous cell carcinoma. We carried out this study to assess the level of oxidative stress in oral cancer patients with various clinical stages.
Blood samples of 48 adult male oral cancer patients with various clinical stages of oral cancer (stage II to stage IV, 16 of each) and 16 age and sex matched healthy subjects were collected. Plasma and erythrocytes levels of thiobarbituric acid reactive substances (TBARS), vitamin E, reduced glutathione (GSH), and activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) were assayed using specific colorimetric methods. The statistical comparisons were performed by one way analysis of variance (ANOVA) followed by Student's t-test.
Elevated lipid peroxidation and decline in non-enzymatic and enzymatic antioxidants status were noticed in oral cancer patients as compared to healthy subjects. The TBARS levels were gradually increased whereas antioxidants were gradually reduced from stage II to stage IV of oral cancer patients.
INTERPRETATION & CONCLUSION: The altered lipid peroxidation in plasma and erythrocytes of oral cancer patients may be related to their compensatory changes in the antioxidants defense system.
脂质过氧化副产物的过度产生以及抗氧化防御系统的紊乱与包括口腔癌在内的多种疾病的发病机制有关。尽管已经对口腔癌患者的脂质过氧化水平和抗氧化剂进行了多项研究，但尚无关于口腔鳞状细胞癌不同临床分期患者的报道。我们开展这项研究以评估不同临床分期口腔癌患者的氧化应激水平。
收集48例不同临床分期（II期至IV期，各16例）的成年男性口腔癌患者以及16例年龄和性别匹配的健康受试者的血样。采用特定比色法检测血浆和红细胞中硫代巴比妥酸反应性物质（TBARS）、维生素E、还原型谷胱甘肽（GSH）的水平，以及超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）的活性。通过单因素方差分析（ANOVA），然后进行Student's t检验进行统计学比较。
与健康受试者相比，口腔癌患者脂质过氧化升高，非酶和酶抗氧化剂状态下降。口腔癌患者从II期到IV期，TBARS水平逐渐升高，而抗氧化剂逐渐减少。
口腔癌患者血浆和红细胞中脂质过氧化的改变可能与其抗氧化防御系统的代偿性变化有关。
