Panjamurthy Kuppusamy, Manoharan Shanmugam, Ramachandran Cinnamanoor Rajamani
Department of Biochemistry, Faculty of Science, Annamalai University, Annamalai Nagar - 608002, India.
Cell Mol Biol Lett. 2005;10(2):255-64.
Our aim was to assess the degree of oxidative stress in patients with periodontitis by measuring their levels of thiobarbituric acid reactive substances (TBARS), enzymatic antioxidants (superoxide dismutase (SOD), catalase (CAT), glutathione peroxide (GSHPx)), and non-enzymatic antioxidants (vitamins E and C, reduced glutathione (GSH)). This study was conducted on 25 adult chronic periodontitis sufferers who were patients in Rajah Muthiah Dental College and Hospital, Annamalai University. The levels of TBARS and non-enzymatic antioxidants, and the activities of enzymatic antioxidants in the patients' plasma, erythrocytes and gingival tissues were assayed using specific colorimetric methods. The periodontitis sufferers had a significantly higher TBARS level than the healthy subjects. In the plasma, erythrocytes, erythrocyte membranes and gingival tissues of the periodontitis sufferers, enzymatic antioxidant activities were found to be significantly higher, whereas the levels of non-enzymatic antioxidants were significantly lower (except for reduced glutathione in the gingival tissues) relative to the parameters found for healthy subjects. The disturbance in the endogenous antioxidant defense system due to over-production of lipid peroxidation products at inflammatory sites can be related to a higher level of oxidative stress in patients with periodontitis.
我们的目的是通过测量牙周炎患者的硫代巴比妥酸反应性物质（TBARS）水平、酶促抗氧化剂（超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSHPx））以及非酶促抗氧化剂（维生素E和C、还原型谷胱甘肽（GSH））来评估其氧化应激程度。本研究对25名成年慢性牙周炎患者进行，这些患者均为安纳马莱大学拉贾·穆西亚牙科学院及医院的病人。采用特定的比色法测定患者血浆、红细胞和牙龈组织中TBARS和非酶促抗氧化剂的水平以及酶促抗氧化剂的活性。牙周炎患者的TBARS水平显著高于健康受试者。相对于健康受试者的参数，在牙周炎患者的血浆、红细胞、红细胞膜和牙龈组织中，酶促抗氧化剂活性显著更高，而非酶促抗氧化剂水平显著更低（牙龈组织中的还原型谷胱甘肽除外）。炎症部位脂质过氧化产物过度产生导致内源性抗氧化防御系统紊乱，这可能与牙周炎患者较高的氧化应激水平有关。
