基于亚麻籽的唾液替代品及其对体外预脱矿牛牙本质矿物质溶解的影响。

Linseed based saliva substitutes and their effect on mineral dissolution of predemineralized bovine dentin in vitro.

作者信息

Meyer-Lueckel Hendrik, Tschoppe Peter, Kielbassa Andrej M

机构信息

Department of Operative Dentistry and Periodontology, University School of Dental Medicine, Campus Benjamin Franklin, Charité, Universitätsmedizin Berlin, Germany.

出版信息

J Dent. 2006 Nov;34(10):751-6. doi: 10.1016/j.jdent.2006.01.006. Epub 2006 Mar 7.

DOI:10.1016/j.jdent.2006.01.006

PMID:16519983

Abstract

OBJECTIVES

Polymers used in saliva substitutes might inhibit remineralization. The present study evaluated the effects of various calcium, phosphate, and fluoride concentrations of linseed based solutions on the mineral loss of predemineralized bovine dentin in vitro.

METHODS

Dentin specimens were prepared, embedded and polished. The specimens were demineralized (pH 5.6; 6 d; 37 degrees C) and half of each sample was covered with nail varnish. Subsequently, the specimens (n=10) were exposed to 14 linseed based solutions (calcium 0-1.1 mM; phosphate 0-7.7 mM, fluoride 0-0.03 mM) at pH 5.5 and 6.5 as well as to Salinum for 14 days (37 degrees C). The differences in mineral loss (DeltaDeltaZ) between the values prior to and after storage in the solutions were evaluated from microradiographs of thin sections.

RESULTS

After the exposure to the various solutions decreased mineral contents compared to those after demineralization could be observed (p<0.05; paired t-test) whereas for the specimens stored in solution with the highest calcium and phosphate concentration no significant change in mineral loss could be evaluated (p>0.05). Salinum induced a significantly greater mineral loss compared to the equivalent saturated solution (p<0.05; ANOVA, Bonferroni). The general linear model revealed a significant dependency for DeltaDeltaZ on 'phosphate' (p=0.000) and 'fluoride' (p=0.001), but not on 'calcium' (p=0.397) and 'pH' (p=0.368).

CONCLUSIONS

The addition of calcium, phosphates, and fluorides seems to have a positive effect on the remineralizing qualities of linseed based saliva substitutes.

摘要

目的

唾液替代品中使用的聚合物可能会抑制再矿化。本研究评估了不同钙、磷和氟浓度的亚麻籽基溶液对体外预先脱矿的牛牙本质矿物质流失的影响。

方法

制备牙本质标本，进行包埋和抛光。将标本脱矿（pH 5.6；6天；37℃），每个样本的一半用指甲油覆盖。随后，将标本（n = 10）暴露于14种亚麻籽基溶液（钙0 - 1.1 mM；磷0 - 7.7 mM，氟0 - 0.03 mM），pH值分别为5.5和6.5，同时暴露于生理盐水14天（37℃）。通过薄片的显微放射照片评估溶液储存前后矿物质流失（ΔΔZ）的差异。

结果

与脱矿后相比，暴露于各种溶液后矿物质含量降低（p < 0.05；配对t检验），而对于储存在钙和磷浓度最高的溶液中的标本，矿物质流失没有显著变化（p > 0.05）。与等效饱和溶液相比，生理盐水导致显著更大的矿物质流失（p < 0.05；方差分析，Bonferroni检验）。一般线性模型显示ΔΔZ对“磷”（p = 0.000）和“氟”（p = 0.001）有显著依赖性，但对“钙”（p = 0.397）和“pH”（p = 0.368）没有依赖性。